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以无籽罗汉果优良株系的幼嫩茎段为试验材料,经消毒处理后,剪成带一个腋芽的茎段,在MS+6-BA0.5 mg·L-1+NAA0.05 mg·L-1培养基上进行培养,获得无菌芽苗,再以无菌芽苗的单芽茎段为外植体,建立无籽罗汉果的组培快繁体系。结果表明,最佳继代增殖培养基为MS+6-BA0.5 mg·L-1+IBA 0.2 mg·L-1+GA30.03 mg·L-1,30 d的增殖系数为16.4;芽苗伸长的最适培养基为MS+6-BA0.05 mg·L-1+IBA 0.1 mg·L-1+GA30.1 mg·L-1;芽苗生根的最适培养基为1/2MS+IBA 0.5 mg·L-1;炼苗后,移入蛭石:珍珠岩:熟土=1:1:2(V/V/V)的基质中,成活率达98.1%。该体系的建立为无籽罗汉果规模化生产提供了技术平台。
The young stem segments of fine seedless Luohanuo lines were used as test materials, and after being disinfected, the stem segments with one axillary buds were cut and the stem segments of the axillary buds with MS + 6-BA 0.5 mg · L -1 + NAA 0.05 mg · L -1 medium to obtain sterile germinated shoots, and then the single bud stem segments of sterile germinated shoots were used as explants to establish a tissue culture system for cultivating seedless Luo Han Guo. The results showed that the best proliferation medium was MS + 6-BA 0.5 mg · L -1 + IBA 0.2 mg · L -1 + GA 30.03 mg · L -1, The optimum culture medium for shoot elongation was MS + 6-BA 0.05 mg · L -1 + IBA 0.1 mg · L -1 + GA 30.1 mg · L -1; 2MS + IBA 0.5 mg · L-1. The survival rate of the medium supplemented with vermiculite and perlite was 1: 1: 2 (V / V / V). The establishment of the system for the seedless Luo Han Guo large-scale production technology provides a platform.