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[Objective] To study the antimicrobial activity of liposoluble compounds from Sargassum pallidum (tvrn) Ag against food spoilage bacteria. [Method] Using the circle agar disc diffusion method, the antimicrobial activity of liposoluble compounds and fractions isolated by column chromatography from S. pallidum was assayed against food spoilage bacteria. [Result] The inhibition zone of S. pallidum crude grease against Escherichia coli and Pseudomonas stutzeri was 1.0 and 1.5 mm. Bacillus subtilis and Staphylococcus aureus were not sensitive to crude grease. Benzene fraction had no antimicrobial activities against Escherichia coli and Pseudomonas stutzeri, and its inhibition zone against Staphylococcus aureus and Bacillus subtilis was both 1.0 mm. Petroleum ether fraction had no antimicrobial activity against 4 food spoilage bacteria. Ethanol fraction showed strong antimicrobial activities against four food spoilage bacteria and the inhibition zone against Bacillus subtilis, Escherichia coli, Staphylococcus aureus and Pseudomonas stutzeri could reach 3.0, 4.5, 4.0, and 2.5 mm. In addition, the blank control group had no antimicrobial activities. [Conclusion] Ethanol fraction of S. pallidum showed strong antimicrobial activities against four food spoilage bacteria. The antimicrobial activities of liposoluble compounds were attributed to their strongly polar lipids.
[Objective] To study the antimicrobial activity of liposoluble compounds from Sargassum pallidum (tvrn) Ag against food spoilage bacteria. [Method] Using the circle agar disc diffusion method, the antimicrobial activity of liposoluble compounds and fractions isolated by column chromatography from S. pallidum [Result] The inhibition zone of S. pallidum crude grease against Escherichia coli and Pseudomonas stutzeri was 1.0 and 1.5 mm. Bacillus subtilis and Staphylococcus aureus were not sensitive to crude grease. Benzene fraction had no antimicrobial activities against Escherichia coli and Pseudomonas stutzeri, and its inhibition zone against Staphylococcus aureus and Bacillus subtilis was both 1.0 mm. Petroleum ether fraction had no antimicrobial activity against 4 food spoilage bacteria. Ethanol fraction showed strong antimicrobial activities against four food spoilage bacteria and the inhibition zone against Bacillus subtilis, Escherichia co Li, Staphylococcus aureus and Pseudomonas stutzeri could reach 3.0, 4.5, 4.0, and 2.5 mm. In addition, the blank control group had no antimicrobial activities. [Conclusion] Ethanol fraction of S. pallidum showed strong antimicrobial activities against four food spoilage bacteria. The antimicrobial activities of liposoluble compounds were attributed to their strongly polar lipids.