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目的:研究不同浓度的MGd1对体外培养胃癌细胞SGC-7901增殖及凋亡的影响。方法:采用MTT法测定不同浓度MGd1对SGC-7901生长抑制作用;流式细胞术(FCM)进行细胞凋亡分析。激光共聚焦显微镜观察MGd1抗原(MGd1-Ag)的亚细胞定位。结果:MTT结果显示不同浓度的MGd1均对SGC-7901细胞产生明显的抑制效应(P=0.02);流式细胞术分析发现MGd1可诱导SGC-7901发生凋亡并呈浓度和时间依赖性(P<0.01);共聚焦显微镜结果显示MGd1-Ag主要定位于细胞膜上。结论:以上结果证实胃癌特异性单抗MGd1可抑制SGC-7901的增殖并促进凋亡发生。它可能通过与细胞膜上抗原特异性结合,影响下游信号传导,从而发挥抑制效应。
Objective: To study the effects of different concentrations of MGd1 on the proliferation and apoptosis of gastric cancer cell line SGC-7901 in vitro. Methods: MTT assay was used to determine the effect of different concentrations of MGd1 on the growth of SGC-7901 cells. Flow cytometry (FCM) was used to analyze the apoptosis of SGC-7901 cells. Subcellular localization of MGd1 antigen (MGd1-Ag) was observed by laser confocal microscopy. Results: MTT assay showed that MGd1 at different concentrations had a significant inhibitory effect on SGC-7901 cells (P = 0.02). Flow cytometry analysis showed that MGd1 induced apoptosis in SGC-7901 cells in a concentration- and time-dependent manner <0.01). The results of confocal microscopy showed that MGd1-Ag mainly localized on the cell membrane. Conclusion: These results confirmed that gastric cancer-specific monoclonal antibody MGd1 can inhibit SGC-7901 proliferation and promote apoptosis. It may exert its inhibitory effect by specifically binding antigen on the cell membrane and affecting downstream signaling.