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应用免疫组化ＬＳＡＢ法，对５０例原发性肝癌、癌周肝组织及１０例正常肝组织中ｃ－ｅｒｂＢ－２、Ｈ－ｒａｓｐ２１和ｐ５３癌基因蛋白及ＰＣＮＡ表达进行比较研究。结果表明，ｃ－ｅｒｂＢ－２、Ｈ－ｒａｓｐ２１、ｐ５３及ＰＣＮＡ阳性表达率，在癌组织中分别为７０．０％、６８．０％、４６．０％及８２．０％；在癌周肝组织中分别为２２．０％、６４．０％、４．０％及５２．０％；正常肝组织中（除Ｈ－ｒａｓｐ２１外）未见表达，且癌组织与非癌组织（Ｈ－ｒａｓｐ２１除外）及正常肝组织之间有显著差异（Ｐ＜０．０５）。ｃ－ｅｒｂＢ－２和ｐ５３蛋白的表达与原发性肝癌的组织学分级有密切关系（Ｐ＜０．０５），且二者的表达有高度的相关性（Ｐ＜０．００５），在癌、癌周肝组织中，２种以上癌基因蛋白同时表达总率分别为１４４．０％、１２．０％，癌基因蛋白与ＰＣＮＡ同时表达总率分别为１５０．０％、８４．０％。提示肝癌的发生是由多种癌基因独立并协同作用伴有细胞异常增殖的恶性转化过程。
The immunohistochemical LSAB method was used to compare the expression of c-erbB-2, H-rasp21 and p53 oncoproteins and PCNA in 50 cases of primary liver cancer, liver tissue around the cancer and 10 cases of normal liver tissues. The results showed that the positive expression rates of c-erbB-2, H-rasp21, p53, and PCNA were 70.0%, 68.0%, 46.0%, and 82.0%, respectively, in cancer tissues; 22.0%, 64.0%, 4.0%, and 52.0% in tissues; no expression in normal liver tissues (except H-rasp21), and cancerous tissues and non-cancer tissues (H-rasp21 Except) and normal liver tissue were significantly different (P<0.05). The expression of c-erbB-2 and p53 protein was closely related to the histological grade of primary liver cancer (P<0.05), and the expression of c-erbB-2 and p53 was highly correlated (P<0.005). The total expression rate of two or more oncogene proteins was 144.0% and 12.0%, respectively. The total expression rate of oncogene protein and PCNA was 150.0% and 84.0%, respectively. It is suggested that the occurrence of hepatocellular carcinoma is a malignant transformation process in which several oncogenes are independent and synergistically associated with abnormal cell proliferation.