论文部分内容阅读
目的研究吡咯列酮对大鼠肾缺血再灌注损伤的保护作用机制。方法大鼠30只随机分为5组:假手术组,缺血再灌注组,吡格列酮5 mg/kg组,吡格列酮10 mg/kg组和吡格列酮20 mg/kg组,每组6只,采用在体左肾动脉钳夹暂时阻断肾血流法制备大鼠肾缺血再灌注模型,取大鼠左肾组织,检测细胞凋亡、8-羟基脱氧鸟苷(8-OHdG)、丙二醛(MDA)、超氧化物歧化酶(SOD)、p53、Bax、Bcl-2和半胱氨酸天冬酶9、3(Caspase 9和Caspase3)的变化。结果与缺血再灌注组相比,吡格列酮5、10和20 mg/kg可呈剂量依赖性减少8-OHdG和MDA含量,降低p53和Bax蛋白表达,抑制Caspase 9和Caspase3酶活性,增加SOD酶活性和Bc-l2蛋白表达,抑制细胞凋亡。结论吡格列酮预处理可降低大鼠肾缺血再灌注后细胞的氧化损伤水平,下调凋亡相关蛋白表达,减少肾细胞凋亡数以起到肾保护作用。
Objective To study the protective effect of pyrrolizidine on renal ischemia-reperfusion injury in rats. Methods Thirty rats were randomly divided into 5 groups: sham operation group, ischemia-reperfusion group, pioglitazone 5 mg / kg group, pioglitazone 10 mg / kg group and pioglitazone 20 mg / kg group, Left renal artery clamp temporarily blocked renal blood flow in rat renal ischemia-reperfusion model, left kidney tissue was taken for detecting apoptosis, 8-OHDG, MDA MDA, SOD, p53, Bax, Bcl-2 and caspase 9,3 (Caspase 9 and Caspase3) were measured. Results Compared with ischemia-reperfusion group, pioglitazone 5, 10 and 20 mg / kg decreased the content of 8-OHdG and MDA, decreased the expression of p53 and Bax protein, inhibited the activity of Caspase 9 and Caspase 3 and increased the activity of SOD Activity and Bc-l2 protein expression, inhibition of apoptosis. Conclusion Pioglitazone preconditioning can reduce the level of oxidative damage, decrease the expression of apoptosis-related protein and decrease the number of renal cell apoptosis after renal ischemia-reperfusion in rats.