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叶片表面茸毛是水稻形态学特征上的一个重要农艺性状,对水稻的生长及生理特性有着重要的影响.应用叶片具有茸毛特征的水稻品种75-1-127,无茸毛水稻品种明恢63,光身稻品种Lemont,9311分别杂交产生F1及F1自交产生的F2群体对水稻茸毛基因进行遗传学分析,结果表明,水稻茸毛性状为一对细胞核基因控制的显性性状.应用75-1-127/明恢63的F2隐性分离群体,结合分离群体分析法(BSA)和隐性群体分析法(RCA),并通过Mapmaker3.0/MapDraw软件分析,将水稻茸毛基因GL6初步定位在水稻第6号染色体上,位于SSR标记RM20491和RM20547之间,且两标记与该茸毛基因的相对遗传距离分别为7.2和2.2cM.进一步构建大的F2分离群体并同时挖掘新的SSR标记及插入缺失InDel标记用于茸毛基因GL6的精细定位,将茸毛基因GL6精细定位在插入缺失标记InDel-106和InDel-115之间,且两标记与该茸毛基因的相对遗传距离分别为0.3和0.1cM,结合GeneBank数据库分析,在该精细定位区域内,对应粳稻日本晴和籼稻9311的物理距离分别为79和116.82kb,分别注释有7个和8个预测基因,为进一步的基因克隆和功能研究奠定了基础.
Leaf surface hairiness is an important agronomic trait on morphological characteristics of rice, and has an important impact on the growth and physiological characteristics of rice.Using paddy rice variety 75-1-127 with leaf hair characteristics, hairless rice variety Minghui 63, light Genetic analysis of paddy hairy genes in F2 population from cross of F1 progenies and F1 F1 hybrids produced by Lemont strain 9311 showed that the hairiness of rice was a dominant trait controlled by nuclear genes.Application of 75-1-127 / Minghui63 F2 population, combined with segregation population analysis (BSA) and recessive population analysis (RCA), and mapped by Mapmaker3.0 / MapDraw software, On the chromosome, located between SSR markers RM20491 and RM20547, and the relative genetic distances between the two markers and the velvet hair gene were 7.2 and 2.2 cM, respectively.Furthermore, a large F2 segregation population was constructed and new SSR markers and inserted InDel markers For the fine positioning of the hairy gene GL6, the hairy gene GL6 was finely located between the insertion deletion markers InDel-106 and InDel-115, and the relative genetic distances between the two markers and the hairy hair gene were 0 .3 and 0.1cM, respectively. According to the analysis of GeneBank database, the corresponding physical distances between Japonica japonica and indica 9311 in the fine mapping region were 79 and 116.82kb, respectively. Seven and eight predicted genes were annotated respectively for further gene cloning And functional research laid the foundation.