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建立了同时测定螺蛳中7种焦油色素残留量的高效液相色谱法。样品经石油醚脱脂后,无水乙醇-氨水-水溶液(体积比为70∶1∶29)提取,HLB固相萃取小柱净化,采用高效液相色谱-二极管阵列检测器测定,外标法定量。结果表明7,种焦油色素在1.0～40.0μg/mL范围内线性关系良好,相关系数(R2)为0.9992～0.9999,当添加水平为2.0～20.0 mg/kg时,加标回收率为71.3%～117%,相对标准偏差为1.9%～12%。柠檬黄、坚牢绿、赤藓红、荧光桃红、虎红、酸性玫瑰红B的测定低限为2.0 mg/kg,亮蓝为4.0 mg/kg。该方法满足焦油色素残留分析的要求。
A high performance liquid chromatography (HPLC) method for simultaneous determination of seven tar pigment residues in snails was established. Samples were degreased with petroleum ether, extracted with absolute ethanol - aqueous ammonia - water (volume ratio 70:1:29), HLB solid phase extraction cartridges were purified by high performance liquid chromatography-diode array detector and quantified by external standard method . The results showed that there was a good linear relationship between the tar pigment and 1.0 ~ 40.0 μg / mL, the correlation coefficient (R2) was 0.9992 ~ 0.9999. The recoveries of the tar pigment at the addition level of 2.0 ~ 20.0 mg / kg were 71.3% 117%, the relative standard deviation of 1.9% to 12%. The lower limit of determination of lemon yellow, fast green, erythrosine, fluorescent pink, tiger red, and acidic rose B is 2.0 mg / kg and the bright blue is 4.0 mg / kg. The method meets the requirements of tar pigment residue analysis.