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利用黑龙江省科学院自然与生态研究所三江平原湿地生态定位研究站内的模拟氮沉降试验平台,应用Miseq测序技术对细菌16SrDNA进行序列测定和分析,探讨添加氮对三江平原小叶章湿地土壤细菌群落结构的影响。结果表明,与对照相比,添加氮增加土壤细菌群落多样性,低氮(4g·hm~(-2)·a~(-1))时土壤细菌多样性最高,高氮(8g·hm~(-2)·a~(-1))次之。模拟氮沉降导致湿地土壤细菌群落的结构以及丰度发生变化,低氮、高氮和对照地的优势种群均为酸杆菌门(Acidobacteria),变形菌门(Proteobacteria)次之,随着氮沉降的增加,酸杆菌门的丰度逐渐下降,变形菌门的丰度则先升高后降低。外源氮的输入使得土壤的理化性质发生了改变,导致细菌群落的组成以及细菌的相对丰度发生改变。16SrDNA heatmap分析则表明,不同的细菌群落对氮沉降的响应不同。本研究为预测未来大气氮沉降对三江平原湿地土壤微生物的影响以及湿地生态系统的变化提供基础数据和理论参考。
Using Miseq sequencing technique to sequence and analyze 16SrDNA of bacteria in the Sanjiang Plain wetland ecological location research station of Heilongjiang Academy of Sciences, and to explore the effect of adding nitrogen on the bacterial community structure in the soil of Calamagrostis angustifolia wetland in Sanjiang Plain. influences. The results showed that, compared with the control, nitrogen was added to increase the diversity of soil bacterial community, and bacterial diversity was the highest under low nitrogen (4 g · hm -2 · a -1) (-2) · a -1 (-1)). The simulated nitrogen deposition led to changes in the structure and abundance of bacterial communities in wetlands. The dominant populations of low nitrogen, high nitrogen and control were Bacteria acidobacteria and Proteobacteria, Increase, the acid abundance of door gradually decreased, the abundance of proteobacteria increased first and then decreased. The input of exogenous nitrogen changed the physical and chemical properties of soil, resulting in the change of the composition of bacterial community and the relative abundance of bacteria. 16SrDNA heatmap analysis showed that different bacterial communities respond differently to nitrogen deposition. This study provides the basic data and theoretical reference for predicting the future impact of atmospheric N deposition on the soil microorganisms and wetland ecosystem in the Sanjiang Plain.