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摘要本研究利用ITS区、tef1基因、cmdA基因和HIS基因4个基因部分序列对2009-2011年间采自云南省主要灰斑病发生区域和吉林省部分区域的玉米灰斑病菌进行比较分析。结果表明，无论是4个基因序列单独聚类还是拼接序列聚类均把云南省灰斑病发生区域采集的菌株与Cercospora zeina聚为一群，而吉林省采集的灰斑病菌则与Cercospora zeaemaydis聚为一群。聚类分析还表明云南省不同玉米产区的灰斑病菌遗传背景单一，在所有聚类树中均聚为一群，且自举支持值均>99%。
关键词玉米灰斑病；分子鉴定；基因序列比对
中图分类号:S 435.13文献标识码：ADOI：10.3969/j.issn.05291542.2014.02.006Molecular identification of maize grey leaf spot pathogens in Yunnan ProvinceHuang Huichuan1,Yang Min1,Wang Yan2,He Xiahong1,Li Chengyun1,Zhu Youyong1,Zhu Shusheng1(1. Key Laboratory of Agriculture Biodiversity for Plant Disease Management, Ministry of Education, Yunnan Agricultural
University, Kunming650201, China; 2. Department of Plant Science, Jilin University, Changchun130012, China)AbstractMonoconidial isolates of the fungus causing gray leaf spot of maize were obtained from diseased leaves collected throughout the Yunnan Province and part area of Jilin Province and analyzed for genetic variability with the method of cluster analysis. The DNA sequences for the internal transcribed spacers (ITS1
关键词玉米灰斑病；分子鉴定；基因序列比对
中图分类号:S 435.13文献标识码：ADOI：10.3969/j.issn.05291542.2014.02.006Molecular identification of maize grey leaf spot pathogens in Yunnan ProvinceHuang Huichuan1,Yang Min1,Wang Yan2,He Xiahong1,Li Chengyun1,Zhu Youyong1,Zhu Shusheng1(1. Key Laboratory of Agriculture Biodiversity for Plant Disease Management, Ministry of Education, Yunnan Agricultural
University, Kunming650201, China; 2. Department of Plant Science, Jilin University, Changchun130012, China)AbstractMonoconidial isolates of the fungus causing gray leaf spot of maize were obtained from diseased leaves collected throughout the Yunnan Province and part area of Jilin Province and analyzed for genetic variability with the method of cluster analysis. The DNA sequences for the internal transcribed spacers (ITS1