论文部分内容阅读
Aim:To determine the androgenic effects of Basella alba and Hibiscus macranthus extracts in the rat and the bull,and to develop a novel in vitro test system using Leydig cells from bull testes.Methods:The effect of methanolextracts from both plants on testosterone producti’on in isolated Leydig cells from the rat and the bull was analyzedusing ~(125)I-radioimmunoassay (~(125)I-RIA).Rat Leydig cells were obtained by common methods,whereas a noveltechnique was used to purify Leydig cells from bull testes.Results:Bull testes from the slaughter house were acheap source of pure Leydig cells.In culture,these cells produced testosterone for 5-6 days,which can be stimulatedby human chorionic gonadotrophin (hCG).Basella alba extracts significantly enhanced testosterone production inbull and rat Leydig cells in a concentration-dependent manner.Hibiscus macranthus showed no androgenic effect butwas shown to inhibit testosterone production at higher concentrations.Conclusion:Leydig cells purified from bulltestes can be used as an alternative tool in experimental animal research.Certain fractions of Basella alba extractdemonstrated androgenic potential whereas Hibiscus macranthus extracts did not.(Asian J Androl 2005 Dec;7:411-417)
Aim:To determine the androgenic effects of Basella alba and Hibiscus macranthus extracts in the rat and the bull, and to develop a novel in vitro test system using Leydig cells from bull testes.Methods:The effect of methanolextracts from both plants on testosterone producti’ On in isolated Leydig cells from the rat and the bull was analyzed using ~(125)I-radioimmunoassay (~(125)I-RIA).Rat Leydig cells were obtained by common methods,whereas a noveltechnique was used to purify Leydig cells from bull testes.Results:Bull testes from the slaughter house were acheap source of pure Leydig cells.In culture,these cells produced testosterone for 5-6 days,which can be stimulated by human chorionic gonadotrophin (hCG).Basella alba extracts significantly enhanced testosterone production inbull单词 and rat Leydig cells in a concentration-dependent manner.Hibiscus macranthus showed no androgenic effect butwas shown to inhibit testosterone production at higher concentrations.Conclusion:Leydig cells purified From bulltestes can be used as an alternative tool in experimental animal research.Certain fractions of Basella alba extract demonstrative androgenic potential only Hibiscus macranthus extracts did not. (Asian J Androl 2005 Dec;7:411-417)