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肝素是抗凝血药,广泛用于治疗心肌梗塞和血管栓塞性疾病。由于肝素的结构和分子量并不固定,目前评价肝素采用抗凝血活性,根据其延长羊血浆再钙化凝固时间(recalcification clotting time,RCT的能力,即羊血浆加钙离子后凝固的时间),借助仪器和目测。本文介绍用激光浊度法测定肝素活性,并与美国药典法作了比较。仪器和试剂:激光浊度仪的比色槽改造成外部可用37±0.5℃水循环。肝素钠(USP标准)、氯化钠、氯化钙和冷冻羊血浆。标准
Heparin is an anticoagulant and is widely used in the treatment of myocardial infarction and vascular embolism. Due to the fact that the structure and molecular weight of heparin are not fixed, heparin is currently evaluated for its anticoagulant activity and based on its ability to recalcify the clotting time (RCT, ie, the time of coagulation of sheep plasma plus calcium ions) Instrument and visual inspection. This article describes the determination of heparin activity by laser turbidimetry, and compared with the United States Pharmacopoeia. Instruments and Reagents: The colorimetric cell of the laser turbidimeter is adapted to an external water cycle of 37 ± 0.5 ° C. Heparin sodium (USP standard), sodium chloride, calcium chloride, and frozen sheep plasma. standard