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目的:探讨氧化应激在吸烟相关喉鳞状细胞癌发生发展中的影响,以及过氧化氢酶(CAT)对吸烟相关喉鳞状细胞癌的临床诊断意义。方法:收集吸烟人群119例,其中吸烟相关喉鳞状细胞癌组68例、非喉鳞状细胞癌人群(对照组)51例,比较2组血浆和癌组织的CAT、丙二醛(MDA)、超氧化物歧化酶(SOD)、还原型谷胱甘肽(GSH)、一氧化氮(NO)水平,分析氧化应激指标与吸烟相关喉鳞状细胞癌及其严重程度的关系。结果:1与对照组相比,喉鳞状细胞癌组的吸烟密度、吸烟支数、CAT、MDA、GSH显著升高(均P<0.01);而SOD、NO明显下降(均P<0.01)。2随着喉鳞状细胞癌组织分化程度越低,血清CAT越高(P<0.01),癌组织匀浆的CAT、MDA、GSH也越高(均P<0.01),而血清NO则越低(P<0.01),癌组织匀浆的SOD、NO越低(均P<0.01);喉鳞状细胞癌患者临床分期越高,血清和癌组织的CAT、癌组织的GSH越高(均P<0.01),而癌组织NO越低(P<0.01)。3血清CAT、MDA为吸烟相关喉鳞状细胞癌发病的独立危险因素(OR=1.060,2.475;P<0.01,P<0.05)。结论:氧化应激是吸烟人群发生喉鳞状细胞癌的主要影响因素,CAT可作为吸烟相关喉鳞状细胞癌的临床诊断指标。
Objective: To investigate the effect of oxidative stress on the occurrence and development of smoking-related laryngeal squamous cell carcinoma and the clinical significance of catalase (CAT) in smoking-related laryngeal squamous cell carcinoma. Methods: A total of 119 smokers were enrolled in this study. Among them, smoking-related laryngeal squamous cell carcinoma (68 cases) and non-laryngeal squamous cell carcinoma (51 cases) were compared. CAT and MDA in plasma and cancer tissue were compared between two groups , Superoxide dismutase (SOD), reduced glutathione (GSH) and nitric oxide (NO) were measured. The relationship between oxidative stress and smoking-related laryngeal squamous cell carcinoma and their severity were analyzed. Results: 1 Compared with the control group, the smoking density, the number of smoking cigarettes, CAT, MDA and GSH in laryngeal squamous cell carcinoma were significantly increased (all P <0.01) . 2 With the lower degree of differentiation of laryngeal squamous cell carcinoma, the serum CAT was higher (P <0.01), the higher the CAT, MDA and GSH were (P <0.01), while the lower the serum NO (P <0.01). The lower the serum SOD and NO were (P <0.01). The higher the clinical stage of laryngeal squamous cell carcinoma was, the higher the serum CAT and the GSH of cancer tissues were (P <0.01), while the lower the NO of cancer tissue (P <0.01). Serum CAT and MDA were independent risk factors of smoking related laryngeal squamous cell carcinoma (OR = 1.060,2.475; P <0.01, P <0.05). Conclusion: Oxidative stress is the main influencing factor of laryngeal squamous cell carcinoma in smokers. CAT can be used as a clinical diagnostic indicator of smoking-related laryngeal squamous cell carcinoma.