论文部分内容阅读
参麦注射液是一种中药复合物,广泛应用于癌症患者的辅助治疗中。在生理(PH7.4)环境下,通过荧光光谱与紫外吸收光谱研究了参麦注射液与人血清白蛋白的相互作用。荧光光谱和紫外吸收光谱实验结果表明,参麦注射液能够有效地引起人血清白蛋白的内荧光源淬灭,其淬灭机理为动态淬灭。利用Stern-Volmer方程对荧光光谱数据进行分析,得到不同温度(296,303,310 K)下的结合常数(KA)。通过Van′t Hoff方程计算出热力学参数(ΔG<0,ΔH>0,ΔS>0),该结果表明疏水力是人血清白蛋白与参麦注射液结合时的主要相互作用力及结合过程是一个自发过程。此外,同步荧光光谱实验结果表明,当参麦注射液与人血清白蛋白结合时,主要结合点位于酪氨酸残基,且引起了人血清白蛋白的结构变化。
Shenmai injection is a traditional Chinese medicine compound, widely used in adjuvant treatment of cancer patients. In physiological (PH7.4) environment, the interaction between Shenmai injection and human serum albumin was studied by fluorescence and UV absorption spectroscopy. Fluorescence spectra and UV absorption spectra show that Shenmai injection can effectively quench the internal fluorescence source of human serum albumin and its quenching mechanism is dynamic quenching. Fluorescence spectra data were analyzed using the Stern-Volmer equation and the binding constants (KA) at different temperatures (296, 303, 310 K) were obtained. The thermodynamic parameters (ΔG <0, ΔH> 0, ΔS> 0) were calculated by the Van’t Hoff equation. The results show that the hydrophobic force is the major interaction force between human serum albumin and Shenmai injection and the binding process is A spontaneous process. In addition, the experimental results of synchronous fluorescence spectroscopy showed that when Shenmai injection was combined with human serum albumin, the major binding sites were located in tyrosine residues and caused the structural change of human serum albumin.