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为探讨水杨酸SA对太子参叶斑病的诱抗效果以及有关酶活性的变化与SA抗病作用的可能联系,在离体培养条件下,运用水杨酸0.5-15 mmol·L~(-1) 6种不同浓度处理太子参组培苗,在1-7d内测定叶片相关酶活性的变化,诱导培养7d后,用太子参叶斑病孢子悬浮液对组培苗进行抗性鉴定。结果表明,SA5 mmol·L~(-1)诱导处理后能明显增强太子参叶片内POD、PPO酶活性,降低CAT酶活性,其中POD活性比对照增加170%,PPO活性比对照增加293%,CAT活性比对照降低43.3%。6种浓度均能不同程度地诱导太子参对叶斑病产生抗性,以5.0 mmol·L~(-1)水杨酸的诱抗效果最好,达到59.6%,诱抗效果极显著高于其它处理浓度。
In order to investigate the inducing effect of salicylic acid (SA) on leaf spot disease of Pseudostellaria heterophylla and the possible relationship between the change of enzyme activity and the disease resistance of SA, the effects of salicylic acid 0.5-15 mmol·L ~ (-1) -1), and the changes of enzyme activities related to the leaf were measured within 1-7 days. After induced for 7 days, the tissue culture seedlings were identified with the spore suspension of Pseudostellaria Leaf spot. The results showed that POD and PPO activities and POD activities in Pseudostellaria Gigantea leaves were significantly increased after SA 5 mmol·L -1 treatment. POD activity increased 170% and PPO activity increased 293% CAT activity decreased by 43.3% compared with the control. All the six concentrations induced the resistance of leaf ginseng to different degrees, and the best induction effect was 5.0 mmol·L -1 salicylic acid (59.6%), the induction effect was significantly higher than that of Other treatment concentrations.