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目的:探讨HLA-B27、抗链球菌溶血素“O”(ASO)、类风湿因子(RF)和C反应蛋白(CRP)检测在强直性脊柱炎(AS)诊断与鉴别诊断中的意义。方法:抽取EDTA(K2)抗凝血2 ml,采用酶联免疫吸附试验法测定HLA-B27,采用免疫透射比浊法检测RF、CRP,采用颗粒增强免疫透射比浊法测定ASO。结果:31例正常健康者HLA-B27检测结果均为阴性,ASO、RF、CRP检测结果在正常范围。108例疑似AS患者中,HLA-B27阳性率为30.6%(33/108)。33例HLA-B27阳性患者中,ASO阳性率为6.1%(2/33),RF阳性率为3.0%(1/33),CRP阳性率为12.1%(4/33)。结论:HLA-B27的检测可提示AS诊断,特别是在不典型AS诊断中具有重要的临床价值。ASO、RF检测可用于AS的鉴别诊断。AS患者HLA-B27阳性者中,其RF、CRP、ASO也可增高。
Objective: To investigate the significance of the detection of HLA-B27, anti-streptolysin “”, rheumatoid factor (RF) and C-reactive protein (CRP) in the diagnosis and differential diagnosis of ankylosing spondylitis . Methods: EDTA (K2) was used to extract anticoagulant blood of 2 ml. HLA-B27 was detected by enzyme-linked immunosorbent assay. RF and CRP were measured by immunoturbidimetric assay and ASO by particle-enhanced immuno-turbidimetry. Results: The results of HLA-B27 test in all 31 normal controls were negative, and the results of ASO, RF and CRP were in the normal range. 108 cases of suspected AS patients, the positive rate of HLA-B27 was 30.6% (33/108). Among 33 HLA-B27 positive patients, the positive rate of ASO was 6.1% (2/33), the positive rate of RF was 3.0% (1/33) and the positive rate of CRP was 12.1% (4/33). Conclusion: The detection of HLA-B27 may be valuable in diagnosing AS, especially in the diagnosis of atypical AS. ASO, RF detection can be used for the differential diagnosis of AS. In patients with HLA-B27-positive AS, its RF, CRP, ASO can also be increased.