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以杂交稻 (汕优 63)为试验材料 ,在木村B营养液中培养至三叶期 ,用草酸 5mmol/L预处理水稻 2d ,再处以氧化胁迫 (用 0 .1mmol/L浓度的活性氧诱发剂甲基紫精处理 )。结果表明 :MV诱发的氧化胁迫下 ,Rubisco及其它可溶性蛋白快速降解。草酸预处理可明显缓解Rubisco及其它可溶性蛋白的降解 ,降解速率分别降低 1 /3和 1 /2左右。植株经草酸处理后其叶片中几种抗氧化酶如AsA POD、SOD、CAT活性大大提高 ,这可能是草酸预处理可缓解氧化胁迫下Rubisco和其它可溶性蛋白降解的重要原因。既然草酸能有效地诱导植物的抗氧化防卫反应 ,它可能作为一种诱抗剂来提高植物的抗逆性
The hybrid rice (Shanyou 63) was used as the experimental materials, and cultivated to three-leaf stage in Kimura B nutrient solution. Rice was pretreated with oxalic acid (5mmol / L) for 2 days and then oxidative stress (induced by 0.1mmol / L H2O2 Methyl viologen treatment). The results showed that Rubisco and other soluble proteins degraded rapidly under MV-induced oxidative stress. Oxalic acid pretreatment can obviously alleviate the degradation of Rubisco and other soluble proteins, and the degradation rate is reduced by about 1/3 and 1/2, respectively. The activities of several antioxidant enzymes such as AsA POD, SOD and CAT in the leaves of plants treated with oxalic acid were greatly increased, which may be the important reason that oxalic acid pretreatment can relieve the degradation of Rubisco and other soluble proteins under the oxidative stress. Since oxalic acid can effectively induce plant antioxidant defense response, it may be used as an inducer to improve the plant’s resistance