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本研究建立中国健康成人外周血B淋巴细胞各种免疫标志的参考范围 ,为临床正确诊断B淋巴细胞增殖性疾病提供基础信息。采用全血溶RBC法和SSC/CD19设门技术以三色流式细胞术对来自 4 1名健康成人EDTA K2 抗凝静脉血进行免疫表型分析。结果表明 :外周血B淋巴细胞几乎全部表达CD2 2 ,CD2 0 ,CD6 2L ,CD4 0 ,CD2 4 ,CD79b ,CD79a和FMC 7分子 ,几乎不表达CD11a ,CD80 ,CD10 3,CD10 ,CD4 0L ,CD5 4 ,CD95L ,CD86和CD95分子 ,CD18,CD4 4 ,CD2 3,CD5 ,CD11c和CD4 3的阳性率各不相同 ,78%的B细胞为IgD阳性、κ/λ比例为 1.2 6。结论 :为了对患者的免疫分型资料进行准确判读和对B淋巴细胞增殖性疾病作出正确的诊断 ,必须考虑正常B细胞群体中各种标志的表达情况。同时 ,在临床工作中不宜直接套用国外的标准 ,而必须注意中国人和西方人群之间在某些B细胞标志方面可能存在的差异。
This study established a reference range of various immune markers of peripheral blood B lymphocytes in healthy Chinese adults and provided the basic information for the correct diagnosis of B lymphoproliferative diseases in clinic. Immunophenotyping of EDTA K2 anticoagulated venous blood from 41 healthy adults was performed by tri-color flow cytometry using whole blood-soluble RBC and SSC / CD19 portal techniques. The results showed that CD2 2, CD20, CD6 2L, CD4 0, CD2 4, CD79b, CD79a and FMC 7 molecules were almost completely expressed in peripheral blood B lymphocytes, but scarcely express CD11a, CD80, CD103, CD10, CD4OL, CD5 4, The positive rates of CD95L, CD86 and CD95 molecules, CD18, CD4 4, CD2 3, CD5, CD11c and CD4 3 were different. 78% of the B cells were IgD positive and the κ / λ ratio was 1.2 6. CONCLUSIONS: In order to accurately interpret the patient’s immunophenotypic data and to correctly diagnose B lymphoproliferative disorders, the expression of various markers in the normal B-cell population must be considered. In the meantime, it is not appropriate to directly apply foreign standards in clinical work, but we must pay attention to the possible differences between Chinese and western populations in some B cell markers.