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目的:建立柴胡属植物地上部分中槲皮素、异鼠李素、山奈素HPLC的含量测定方法。方法:伊利特-C18柱(250 mm×4．6 mm,5μm),以甲醇-水(60:40),磷酸调节pH至3．0为流动相,流速:1．0 ml·min-1,检测波长:360 nm。结果:槲皮素在0．70-5．57μg(r槲=0．999 7),异鼠李素在0．16～1．30μg(r异=0．999 2),山奈素在0．09-0．71μg(r山=0．999 4)范围内呈良好的线性关系。平均回收率分别为97．0％,96．8％,97．4％,RSD分别为2．2％,2．2％,1．1％。结论:本法专属性强,灵敏度高,重现性好,适于黄酮类成分的分离与测定。
Objective: To establish an HPLC method for the determination of quercetin, isorhamnetin and kaempferol in aerial parts of Bupleurum plants. Method: Elite-C18 column (250 mm×4.6 mm, 5 μm) with methanol-water (60:40), pH adjusted to 3.0 with mobile phase of phosphoric acid, flow rate: 1.0 ml·min-1 , Detection wavelength: 360 nm. RESULTS: Quercetin was in the range of 0.70-5.57 μg (r槲=0.999 7), isorhamnetin 0.16-1.30 μg (r=0.999 2), and kaempferol at 0. There is a good linear relationship within the range of 09-0.71 μg (r mountain=0.999 4). The average recoveries were 97.0%, 96.8%, and 97.4%, respectively, and the RSDs were 2.2%, 2.2%, and 1.1%, respectively. Conclusion: This method is highly specific, sensitive, and reproducible. It is suitable for the separation and determination of flavonoids.