论文部分内容阅读
HRP—CB标记结合sholl分析方法分析研究5、13、24月龄三个年龄组C57BL/6J小鼠脊髓前角α—运动神经元树突结构的可塑性变化,以及不同时间的长期适量运动(跑转笼)对运动神经元树突结构可塑性变化的可能作用。以同年龄对照组心重/体重比率均值的二倍标准差(X+2SD)做为运动有效标准。结果在三个年龄对照组中,老年鼠(对照组Ⅱ)神经元树突野缩小,树突分支数和总长度明显减少(P<0.05),以远离胞体的分支丢失为主。经过8和19个月运动训练后,脊髓前角运动神经元树突野扩大,分支数和树突总长度明显增加(P<0.01),甚至超过青年对照,以近胞体分支的增生更明显。结果表明,长期的适量运动能够延缓衰老过程中小鼠脊髓前角运动神经元树突的丢失,促进树突的可塑性增生。
HRP-CB combined with sholl analysis was used to analyze the plasticity of dendritic structure of spinal cortex α-motor neurons in C57BL / 6J mice aged 5, 13 and 24 months old, and the long-term moderate exercise at different time Cage) on the plasticity of dendritic structure in motor neurons. The standard deviation (X + 2SD) of the mean of body weight / body weight ratio in the same age group was used as the effective standard of exercise. Results In the three age control groups, the dendritic field of the aged mice (control group Ⅱ) was reduced, the number of dendritic branches and the total length were significantly decreased (P <0.05), and the loss of branches away from the cell body was dominant. After 8 and 19 months of exercise training, the dendritic fields of motor neurons in the anterior horn of the spinal cord were enlarged, the number of branches and total length of dendrites were significantly increased (P <0.01), and even more than the youth controls. The results showed that long-term moderate exercise could delay the loss of dendrites in the anterior horn motor neurons of mice during aging and promote the plasticity of dendrites.