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目的:筛选高效厌氧水解产酸菌,并对其产酸特性进行研究,以提高中药废水厌氧消化水解效率。方法:采用亨盖特厌氧操作技术从中药废水中分离厌氧高效产酸细菌,经形态学、生理生化、16S rDNA基因序列分析确定菌株分类地位,同时对其产酸条件进行研究。结果:经鉴定CD-8为丁酸梭状芽孢杆菌(Clostridium butyricum)。37℃、初始pH 8.0培养具有最大生长及乙酸产量1 148.35 mg/L,不耐盐,培养基中加入8 mmol/L K+能明显促进菌株CD-8的发酵产酸,乙酸产量提高17.69%;能利用葡萄糖、蔗糖、淀粉、甘油等发酵产乙酸,最高达到1 456.48 mg/L。结论:菌株CD-8生长迅速,底物利用范围广,产酸高效稳定。在提高中药废水厌氧消化水解效率、中药渣沼气资源化利用等方面具有潜在的应用价值。
OBJECTIVE: To screen highly efficient anaerobic hydrolytic acid-producing bacteria and study its acidogenicity to improve the anaerobic digestion and hydrolysis efficiency of Chinese traditional medicine wastewater. Methods: The anaerobic and high acidogenic bacterium was isolated from Chinese traditional medicine wastewater by means of the anaerobic operation of Hengert. The morphological, physiological and biochemical, 16S rDNA gene sequence analysis was used to determine the taxonomic status of the strain and the acidogenic conditions were studied. Results: CD-8 was identified as Clostridium butyricum. At 37 ℃, initial pH 8.0, the maximum growth rate and acetic acid yield were 1 148.35 mg / L, and salt tolerance was not observed. Adding 8 mmol / L K + into the medium could significantly promote the fermentation of strain CD-8, and the yield of acetic acid increased by 17.69%. Can use glucose, sucrose, starch, glycerol and other fermentation acetic acid production, up to 1 456.48 mg / L. Conclusion: The strain CD-8 grows rapidly with a wide range of substrate utilization and efficient and stable acid production. It has potential application value in improving the anaerobic digestion and hydrolysis efficiency of traditional Chinese medicine wastewater and the resource utilization of Chinese medicine residue gas.