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目的 研究胶原 -聚羟基乙酸与骨髓间质干细胞的细胞相容性 ,为构建组织工程化肌腱提供实验基础。方法 分离、培养并鉴定骨髓间质干细胞 ,将骨髓间质干细胞置入含胶原 -聚羟基乙酸的DMEM培基中培养 ,设为实验组 ;将骨髓间质干细胞置入纯DMEM培基中培养 ,设为对照组。比较两组细胞的活性和生长情况 ,并对实验组进行超微结构观察。结果 骨髓间质干细胞接种于胶原 -聚羟基乙酸中后可适应材料生长 ,混合培养后 2周生长良好 ,始终保持 89%以上的细胞活力 ,与对照组比较无显著差别 (P >0 0 5 ) ;在 2周的培养过程中实验组细胞数未发生明显改变 ,而对照组从第 4d开始即发生增殖。透射电镜示实验组细胞培养 14d后仍保持旺盛的分泌功能。结论 骨髓间质干细胞与胶原 -聚羟基乙酸的细胞相容性好。以骨髓间质干细胞为种子细胞 ,以胶原 -聚羟基乙酸为支架可在体外预构组织工程化肌腱。
Objective To study the cytocompatibility of collagen-polyglycolic acid and bone marrow mesenchymal stem cells, and provide experimental basis for the construction of tissue-engineered tendon. METHODS: Bone marrow mesenchymal stem cells were isolated, cultured, and cultured in DMEM medium containing collagen-polyglycolic acid. The bone marrow mesenchymal stem cells were cultured in DMEM medium. Set as control group. The activity and growth of the two groups of cells were compared, and the ultrastructure of the experimental group was observed. Results Bone marrow mesenchymal stem cells inoculated with collagen-polyglycolic acid could adapt to the growth of the material. After 2 weeks of culture, BMSCs grew well and maintained more than 89% viability. There was no significant difference compared with the control group (P> 0.05) During the two weeks of culture, the number of cells in the experimental group did not change significantly, while the control group began to proliferate from the 4th day. Transmission electron microscopy showed that the experimental group maintained strong excretory function after 14 days of cell culture. Conclusion BMSCs have good cytocompatibility with collagen-polyglycolic acid. Bone marrow mesenchymal stem cells as seed cells, collagen - polyglycolic acid as a scaffold in vitro pre-organized tissue-engineered tendon.