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目的:探讨PDT法制备的抗小鼠H22肝癌肿瘤疫苗的抗瘤效应。方法:昆明鼠60只,随机分为2组,每组30只。实验组:取6-12周龄的昆明鼠背部皮下接种PDT法产生的疫苗,每3天注射一次,每次注射50μl(相当于3×105个细胞),连续两周。一周以后再注射H22肿瘤细胞悬液0.1ml(1×106个细胞);对照组:每周每次注射50μl生理盐水,连续两周。一周以后再注射H22肿瘤细胞悬液0.1ml(1×106个细胞)。比较两组的抑瘤率和生存率。结果:实验组小鼠的抑瘤率、生存率均较对照组有显著提高。结论:PDT法产生的抗小鼠H22肝癌疫苗具有明显的抗瘤效应,该方法有望成为一种临床治疗肿瘤的新方法。
Objective: To investigate the anti-tumor effect of anti-mouse H22 hepatoma tumor vaccine prepared by PDT. Methods: Kunming mice 60 were randomly divided into two groups of 30. Experimental group: Kunming mice (6-12 weeks old) were inoculated subcutaneously with PDT vaccine and injected every 3 days. Each injection was 50μl (equivalent to 3 × 105 cells) for two weeks. One week later, 0.1 ml H22 tumor cell suspension (1 × 106 cells) was injected. The control group was injected with 50 μl normal saline once a week for two weeks. One week later, 0.1 ml H22 tumor cell suspension (1 x 106 cells) was injected. The tumor inhibition rate and survival rate of the two groups were compared. Results: The inhibitory rate and survival rate of mice in experimental group were significantly higher than those in control group. Conclusion: The anti-tumor effect of H22 hepatocarcinoma vaccine induced by PDT has obvious anti-tumor effect. This method is expected to become a new method of clinical treatment of cancer.