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目的研究miR-200c通过wnt/β-catenin信号通路对结肠癌SW480细胞侵袭、迁移的影响。方法将SW480细胞分为类似物转染(A)组、抑制物转染(B)组、类似物阴性对照(C)组、抑制物阴性对照组(D)和空白(E)组。荧光定量PCR检测12h和24h转染效率,划痕实验和Transwell侵袭实验检测细胞迁移和侵袭,Western blot检测转染后β-catenin和E-cadherin的表达。结果细胞划痕实验显示,A组12h和24h后伤痕宽度分别为(589.61±17.28)μm和(523.83±57.13)μm,C组分别为(465.33±16.60)μm和(393.99±7.53)μm,A组高于C组(P<0.05)。B组12h和24h后分别为(430.93±20.76)μm和(221.38±44.37)μm,D组分别为(485.64±16.65)μm和(441.22±22.40)μm,B组低于D组(P<0.05)。Transwell实验显示,与C组和D组相比,A组24h和48h通过8μm孔径的细胞数明显减少,B组明显增多(P<0.05)。A组β-catenin和E-cadherin的表达均升高,B组各蛋白表达下降(P<0.05)。结论 miR-200c可能通过wnt/β-catenin信号通路抑制SW480结肠癌细胞的侵袭和迁移。
Objective To investigate the effect of miR-200c on invasion and migration of human colon cancer SW480 cells via wnt / β-catenin signaling pathway. Methods SW480 cells were divided into group A (analog), group B (transfected with inhibitor), group C (negative control), group D (negative control) and group E (blank). Fluorescent quantitative PCR was used to detect the transfection efficiency at 12h and 24h. Scratch assay and Transwell invasion assay were used to detect cell migration and invasion. Western blot was used to detect the expression of β-catenin and E-cadherin. Results The cell scratch test showed that the widths of the wounds in the group A were (589.61 ± 17.28) μm and (523.83 ± 57.13) μm at 12h and 24h respectively, and those in the group C were (465.33 ± 16.60) μm and (393.99 ± 7.53) μm, respectively Group than in group C (P <0.05). (430.93 ± 20.76) μm and (221.38 ± 44.37) μm in group B and (485.64 ± 16.65) μm and (441.22 ± 22.40) μm in group B respectively ). Compared with group C and group D, the number of cells passing through 8μm aperture in group A at 24h and 48h was significantly lower than that in group C and group D (P <0.05). The expressions of β-catenin and E-cadherin in group A were increased, and those in group B were decreased (P <0.05). Conclusion miR-200c may inhibit the invasion and migration of SW480 colon cancer cells through the wnt / β-catenin signaling pathway.