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为研究在自然月经周期中获得的人类不成熟卵母细胞 -放射冠 -卵丘细胞复合体体外成熟培养条件 ,作者从手术切除的卵巢组织获取不成熟卵母细胞 -放射冠 -卵丘细胞复合体 ,分别置于 90 % Ham’ F-1 0 + 1 0 %灭活人血清 + FSH+ h CG(模拟卵泡液 )与 5 0 %Ham’ F-1 0 + 5 0 %人类成熟卵泡液中培养 48h。结果显示 ,卵母细胞 -放射冠 -卵丘细胞复合体在两种体外成熟培养基中培养 48h后 ,成熟率分别为 41 .8%和 2 1 .1 % ,两者之间存在统计学差异 (P<0 .0 5 )。作者还比较了在前一种培养基中卵母细胞 -放射冠 -卵丘细胞复合体和裸卵的体外成熟率 ,分别为 41 .8%和 7.6% ,两者间存在统计学差异 (P<0 .0 0 5 )。将获取的不成熟卵母细胞 -放射冠 -卵丘细胞复合体按供者年龄分成 2 5～ 3 5岁组和 3 6～46岁组 ,比较此两组卵母细胞在前一种培养基中成熟率 ,分别为 5 7.1 %和 2 5 .0 % ,两者间在统计学上有显著性差异 (P<0 .0 1 )。结果提示 :不成熟卵母细胞 -放射冠 -卵丘细胞复合体可在模拟卵泡液中培养成熟 ,优于 5 0 % Ham’ F-1 0 + 5 0 %人类成熟卵泡液 ;卵丘细胞包裹对于卵母细胞体外成熟有重要作用 ;供卵者年龄可影响卵母细胞的体外成熟率。
In order to study the in vitro maturation conditions of human immature oocyte-cumulus cell complex obtained in the natural menstrual cycle, the authors obtained immature oocyte-radiation crest-cumulus cell complex from surgically excised ovarian tissue Were cultured in 90% Ham ’F-10 + 10% inactivated human serum + FSH + h CG (simulated follicular fluid) and 50% Ham’ F-10 + 50% human mature follicular fluid 48h. The results showed that the maturation rates of oocyte-radiated corpus cumulus complex were 41.8% and 21.1%, respectively, in two kinds of in vitro maturation medium for 48h, there was a significant difference (P <0. 05). The authors also compared in vitro maturation rates of oocyte-irradiated coronal-cumulus complex and naked egg in the former culture medium with a mean of 41.8% and 7.6%, respectively, with a statistically significant difference (P <0 0 0 5). The obtained immature oocyte-irradiated corona-cumulus complex was divided into 2 5 ~ 3 5 years old group and 3 6 ~ 46 years old group according to donor age. The oocytes of these two groups were compared in the former medium The maturation rates were 51.1% and 25.0%, respectively, with a statistically significant difference (P <0.01). The results suggest that the immature oocyte-irradiated corona-cumulus complex can mature in simulated follicular fluid and is superior to 50% Ham ’F-1 0 + 50% human mature follicular fluid. The cumulus cells For oocyte maturation in vitro has an important role; ovarian age can affect the in vitro maturation rate of oocytes.