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目的研究重庆地区婴幼儿轮状病毒的分子流行病学特征和相关临床资料分析。方法收集2008年10月至2009年2月在重庆儿童医院就诊的患非细菌性急性腹泻的婴幼儿大便和问卷。先用胶体金法检测轮状病毒抗原,并经RT-PCR(逆转录PCR)方法证实为轮状病毒,然后用巢式RT-PCR方法进行VP7(G)、VP4(P)基因分型并分析相关临床资料。结果共收集到507份大便标本,胶体金法检测轮状病毒抗原阳性率50.69%(257/507)。147份轮状病毒VP6基因阳性标本中G分型结果:G1型占40.14%(59/147);G2型6.80%(10/147);G3型17.01%(25/147);G4型2.04%(3/147);G9型0.68%(1/147);尚有49份未能分型,占33.33%(49/147)。P分型结果:P[8]型占60.54%(89/147);P[4]型占6.12%(9/147);P[9]型和P[10]型各有1例,分别占0.68%(1/147);尚有47份未能分型。G、P型别组合最常见的是G1/P[8]型,占58.93%(33/56)。各G、P型别间临床症状比较无统计学差异。结论G、P分型结果与其他地区明显不同,G分型显示出多样性特点,P分型仍以P[8]型占优势。
Objective To study the molecular epidemiology of infants and young children in Chongqing and their clinical data. Methods Totals and questionnaires of infants and young children with non-bacterial acute diarrhea who were treated in Chongqing Children’s Hospital from October 2008 to February 2009 were collected. Rotavirus antigen was detected by colloidal gold method and confirmed as rotavirus by RT-PCR (Reverse Transcription-Polymerase Chain Reaction) method. Then VP7 (G) and VP4 (P) genotypes were detected by nested RT- Analysis of relevant clinical data. Results A total of 507 stool samples were collected. The positive rate of rotavirus antigen by colloidal gold assay was 50.69% (257/507). The results of G typing of 147 rotavirus VP6 gene positive samples showed that G1 type accounted for 40.14% (59/147), G2 type 6.80% (10/147), G3 type 17.01% (25/147), G4 type 2.04% (3/147); G9 type 0.68% (1/147); 49 still failed to type, accounting for 33.33% (49/147). P classification results: P [8] accounted for 60.54% (89/147); P [4] accounted for 6.12% (9/147); P [9] and P [10] Accounting for 0.68% (1/147); there are still 47 failed to type. The most common type G and P type combinations were G1 / P [8], accounting for 58.93% (33/56). The G, P type of clinical symptoms between the no significant difference. Conclusion The results of G and P typing are obviously different from those of other regions. The G typing shows diversified features, and the P typing is still dominated by P [8].