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本研究采用AFLP标记对河北省、辽宁省、吉林省、山西省、内蒙古自治区和西藏自治区的10份野生扁蓿豆居群进行遗传多样性分析。从64对AFLP引物组合中,筛选出8对扩增条带清晰、多态性高的引物组合,8对引物共扩增出640个条带,其中多态性条带有472个,多态性位点百分率为73.8%;Nei’s基因多样性指数平均为0.157,Shannon’s多态性信息指数平均为0.099;居群间遗传相似系数(GS)平均值为0.827。聚类和主成分分析可将10个扁蓿豆居群聚为3大类,结果与居群的地理分布大致相符,呈一定的地域性分布规律。由此可见,AFLP分子标记研究结果能较好地揭示扁蓿豆居群间的遗传多样性。对我国各地的野生扁蓿豆资源的广泛收集、评价和基因资源的保护有重要的意义。
In this study, AFLP markers were used to analyze the genetic diversity of 10 wild Medicago betula populations in Hebei, Liaoning, Jilin, Shanxi, Inner Mongolia Autonomous Region and Tibet Autonomous Region. Eight pairs of primer combinations with clear bands and high polymorphism were screened from 64 pairs of AFLP primer combinations. A total of 640 bands were amplified by 8 pairs of primers, of which 472 were polymorphic bands. Polymorphism The percentage of sexual loci was 73.8%. The average of Nei’s gene diversity index was 0.157 and Shannon’s polymorphism information index was 0.099. The average genetic similarity coefficient (GS) between populations was 0.827. Clustering and principal component analysis of 10 alfalfa beans clustered into three categories, the results generally consistent with the geographical distribution of the population, showing a certain geographical distribution. Thus, the results of AFLP molecular markers can better reveal the genetic diversity among populations of Medicago ruthenica. Extensive collection, evaluation and conservation of genetic resources of wild Medicago alba in various parts of China are of great significance.