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为筛选桃小食心虫高致病性菌株,以在山西省自然感病的桃小食心虫越冬幼虫上分离获得的5株病原真菌为材料,研究各菌株感染桃小食心虫幼虫的症状、对寄主的致死率,以及与菌株毒力相关的胞外类枯草杆菌蛋白酶、几丁质酶和脂肪酶的活性变化,并评价其生物防治潜力。5个菌株在寄主上的感病症状和致死率有显著差异,感病后10天,球孢白僵菌BbTST05对寄主的致死率最高,为89.3%,其类枯草杆菌蛋白酶、几丁质酶和脂肪酶3种酶活性均最大;粉棒束孢IfTSL02对寄主的致死率及其蛋白酶、几丁质酶活性次之;尖孢镰孢菌的FoTSL01、FoTSL03和FoTSL04菌株对桃小食心虫的致死率均低于61%,3种胞外酶的活性也较低。因此,球孢白僵菌BbTST05和粉棒束孢IfTSL02可用于桃小食心虫的防治。
In order to screen the pathogenic bacteria of peach moth, the five pathogenic fungi isolated from overwintering larvae of naturally infected peach moth in Shanxi Province were used as materials to study the symptoms of the host larvae infected with the peach, Rate, and changes in the activity of extracellular subtilisin, chitinase and lipase related to the virulence of the strain, and evaluate their biological control potential. The susceptible symptoms and lethal rates of five strains were significantly different on the host, and the mortality of B. bassiana BbTST05 to host was the highest at 89 days after 10 days of infection. The subtilisin, chitinase And lipase. The lethality and the protease and chitinase activities of host IfTSL02 were the second, and those of Fusarium oxysporum FoTSL01, FoTSL03 and FoTSL04 were the most important Rates were lower than 61%, three kinds of extracellular enzyme activity is also low. Therefore, Beauveria bassiana BbTST05 and IfTSL02 could be used to control peach moth.