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柴胡习用根部入药。但据北京市药品标准办公室统计,有部分省市以带根全草或其茎叶入药,目前广东省就有柴胡根和其茎叶两种商品。柴胡的茎叶是否具有根的功能,尚缺乏依据,为此,我们在化学成分及药理作用方面对柴胡Bupleurum chinense(购自高要县)的根和茎叶进行了比较如下: 一、化学成分方面; (1)柴胡皂甙的比较①柴胡皂甙的含量:精密称取柴胡根(或茎叶)粉末10克,用含2%氢氧化钾甲醇溶液回流提取至提取液无色,合并甲醇液,浓缩至糖浆状,加适量水溶解,过滤,滤液再用水饱和的正丁醇提取。提取液减压浓
Bupleurum is used as a medicine in the roots. However, according to statistics from the Beijing Municipal Bureau of Drug Standards, some provinces and municipalities use roots and whole grass or their stems and leaves for medicine. At present, there are two types of commodities in Guangdong Province: Radix Bupleuri Root and its stem and leaves. Whether the stems and leaves of Bupleurum chinense does not have the function of roots is still unfounded. For this reason, we compared the roots, stems and leaves of Bupleurum chinense (from Gaoyao County) in terms of chemical composition and pharmacological effects as follows: Chemical composition; (1) Comparison of saikosaponin 1 Content of saikosaponin: 10 grams of Radix Bupleurum root (or stem and leaf) powder is precisely weighed, and extracted with a 2% potassium hydroxide solution in methanol to extract the colorless solution. The methanol solution was combined, concentrated to a syrup, dissolved in an appropriate amount of water, filtered, and the filtrate was extracted with n-butanol saturated with water. Extract liquid decompression