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从获得４倍体（亚洲棉×司笃克氏棉）×陆地棉２（Ａ２Ｅ１）×２（ＡＤ）１杂交种子（ＡＤ）１Ａ２Ｅ１到ＢＣ３Ｆ１植株，历时了１０ａ时间。该组合涉及到３个亲本棉种之间的亲缘关系，特别是Ｅ１染色体组与Ａ２和（ＡＤ）１两组之间的亲缘关系较远，导致杂种低世代（Ｆ１和ＢＣ１Ｆ１）雌、雄高度不育。表现在花粉几乎全部败育，发育不正常的胚囊达９０％以上。直至ＢＣ３Ｆ１的群体中还有１０％的不育株。可育株的花中仍有约２５％不育花粉和３５％发育不正常的胚囊。此组合必须经过多次回交、自交及自然恢复过程（越冬），方能最后获得雌、雄育成恢复正常的后代。
The time lasted for a period of 10 a from obtaining the quadruples (Asiatic cotton × Secretary Dickinson cotton) × Upland cotton 2 (ADE) 1A2E1 to BC3F1 hybrid plants. This combination involved the genetic relationship among three parental cotton species. In particular, the genetic relationship between the E1 genome and the A2 and (AD) 1 groups was far, resulting in lower male and female F1-BC1F1 male and female heights Infertility Performance in pollen almost all abortion, abnormal embryo sac up to 90%. Until the population of BC3F1 there is still 10% of the sterile plants. About 25% sterile pollen and 35% dysplastic blastocysts are still present in fertile plants. This combination must undergo multiple backcrossing, selfing and natural recovery processes (overwintering) before the female and male can be finally harvested to restore normal offspring.