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目的观察乌司他丁(UTI)对脓毒症小鼠生存率及淋巴细胞凋亡的影响,探讨UTI对脓毒症的可能作用机制。方法 8～10周C57BL/6雄性小鼠40只,随机分成4组:假手术(Sham)组,盲肠结扎穿孔(CLP)组,CLP+UTI(10万U/kg)术后30 min腹腔内给药(UTI ip)组和CLP+UTI(10万U/kg)术后30 min尾静脉给药(UTI iv)组。在CLP模型建立成功后,每组10只小鼠首先进行7 d生存状况观察研究,描绘7 d生存曲线。另选取40只小鼠按以上方法分组,在CLP术后24 h取材,计数外周血、胸腺和脾脏淋巴细胞,检测胸腺和脾脏淋巴细胞凋亡情况。数据采用Prism5.01(GraphPad Software,USA)软件包进行统计学处理。结果 UTI治疗组7 d生存率高于CLP组,差异有统计学意义(P<0.05)。UTI组外周血、胸腺和脾脏淋巴细胞计数高于CLP组,差异有统计学意义(P<0.05);UTI组脓毒症小鼠胸腺和脾脏淋巴细胞凋亡低于CLP组,差异有统计学意义(P<0.05)。UTI ip和UTI iv组之间各项指标差异均无统计学意义。结论应用UTI腹腔或尾静脉给药可明显改善脓毒症小鼠的生存状况,可能与其升高脓毒症小鼠外周血、胸腺和脾脏淋巴细胞数量,有效降低脓毒症小鼠胸腺和脾脏组织淋巴细胞凋亡等有关。
Objective To investigate the effect of ulinastatin (UTI) on the survival rate and lymphocyte apoptosis in septic mice, and to explore the possible mechanism of UTI on sepsis. Methods Forty C57BL / 6 male mice aged 8 weeks to 10 weeks were randomly divided into 4 groups: sham group, CLP group, CLP + UTI group (30 000 U / kg) (UTI ip) group and CLP + UTI (100000 U / kg) 30 min after tail vein administration (UTI iv) group. After successful establishment of the CLP model, 10 mice in each group were observed for the first 7 days and the 7-day survival curve was drawn. Another 40 mice were divided into groups according to the above methods. The cells were harvested 24 hours after CLP, and the peripheral blood, thymus and spleen lymphocytes were counted. The apoptosis of thymus and spleen lymphocytes was detected. Data were statistically processed using the Prism 5.01 (GraphPad Software, USA) software package. Results The 7-day survival rate of UTI group was significantly higher than that of CLP group (P <0.05). Lymphocyte counts in peripheral blood, thymus and spleen of UTI group were significantly higher than those of CLP group (P <0.05). The apoptosis of thymus and spleen in UTI group was lower than CLP group, the difference was statistically significant Significance (P <0.05). UTI ip and UTI iv group differences in the indicators were not statistically significant. Conclusion Intraperitoneal or tail vein administration of UTI can significantly improve the survival of mice with sepsis, which may be related to the increase in the number of peripheral blood, thymus and spleen lymphocytes in septic mice, effectively reducing the thymus and spleen of septic mice Tissue lymphocyte apoptosis and other related.