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三黄占2号是国际公认、具有稻瘟病持久抗性的籼稻品种。为了获得用于三黄占2号稻瘟病持久抗性基因鉴定及机制研究的突变体,利用化学诱变剂EMS对三黄占2号进行诱变处理,创建突变体库,并对三黄占2号EMS突变体进行稻瘟病抗性评价。以成苗率50%为标准,筛选出最佳EMS诱变浓度为0.8%。从M2代起以单粒传方法进行突变体库的构建,获得了性状相对稳定的7 387份M4代突变体。广东阳江稻瘟病自然诱发鉴定结果表明,约有5.9%和5.4%的材料的叶瘟和穗瘟抗性分别发生了突变,并观察到多种抗性变化类型,从中筛选出300份突变体作进一步的研究。
Sanhuangzhan 2 is an internationally recognized indica rice with long lasting resistance to blast disease. In order to obtain the mutants that were used to identify and study the mechanism of long-lasting resistance to the blast disease of Sanhuangzhan 2, a mutagen was constructed by EMS mutagenesis of Sanhuangzhan No.2, 2 EMS mutants were evaluated for blast resistance. With the seedling rate of 50% as the standard, the optimal EMS mutagenesis concentration was selected as 0.8%. From M2 generation, the mutant library was constructed by single particle transfer method, and 7 387 M4 generation mutants with relatively stable traits were obtained. The natural induced identification of rice blast in Yangjiang, Guangdong Province showed that about 5.9% and 5.4% of the materials were mutated respectively for leaf blast and panicle blast resistance, and a variety of resistant variation types were observed. 300 mutants were screened Further research