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采用HPLC测蔗渣水解液及发酵液中木糖、木糖醇等。在SpherisorbNH2柱上用乙腈+水(80+20)分离,示差折光检测器检测, 15min内同时分离测定木糖、木糖醇及其它五种糖。线性范围在 0~7.5 g/L,相对偏差小于4.1%。
Using HPLC to detect bagasse hydrolyzate and fermentation broth in xylose, xylitol and so on. On SpherisorbNH2 column with acetonitrile + water (80 +20) separation, refractive index detector detection, 15min simultaneous determination of xylose, xylitol and five other sugar. The linear range of 0 ~ 7.5 g / L, the relative deviation of less than 4.1%.