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由于抗生素过量使用已导致我国部分水源受到多种抗生素和耐药基因的污染,抗生素及其耐药基因可能通过饮用水对人体健康造成严重威胁.对上海某水厂供水系统可培养微生物进行了分离鉴定;使用5种抗生素:氨苄青霉素(Amp)、卡那霉素(Kan)、利福平(Rif)、氯霉素(Cm)以及链霉素(Str)对饮用水中可培养微生物多重耐药性及其在供水系统中的变化规律和耐药机制进行了分析.结果表明:所分离可培养微生物主要属于16个属,其中芽孢杆菌属(Bacillus sp.)、噬纤维菌属(Arcicella rosea sp.)、鞘酯菌属(Sphingomonas sp.)在所有工艺单元中都可分离获得;经过生物活性炭滤池和管网输配后3种菌的多重耐药能力均有增强,其中芽孢杆菌属(Bacillus sp.)耐药能力最强.采用实时荧光定量PCR对供水系统中3种整合子及9种转座子进行定量检测,发现经过生物活性炭滤池和管网输配后,可移动基因元件绝对数量增加明显,生物活性炭滤池和输配管网对饮用水中微生物耐药能力具有重要影响.
Due to overuse of antibiotics, some water sources in our country are contaminated by many kinds of antibiotics and drug-resistant genes, and antibiotics and their drug-resistance genes may pose a serious threat to human health through drinking water.Culture of microorganisms in a water supply system of a water plant in Shanghai was separated (Amp), Kan, Rif, Chloramphenicol (Cm) and Streptomycin (Str) were used to evaluate the resistance of multidrug-resistant microorganisms in drinking water The results showed that the cultivated microbes mainly belong to 16 genera, including Bacillus sp., Arcicella rosea sp.) and Sphingomonas sp. were isolated in all the process units. The multi-drug resistance of the three kinds of bacteria increased after bio-activated carbon filter and pipe-line transmission. Among them, Bacillus (Bacillus sp.) Was the most resistant.Using real-time fluorescence quantitative PCR for quantitative determination of three kinds of integron and nine kinds of transposon in the water supply system, it was found that after the transfusion of biological activated carbon filter and pipe network, element The absolute amount increased significantly, and the biological activated carbon filter and transmission pipe network have an important influence on the microbial resistance in drinking water.