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目的探讨谷氨酰-半胱氨酸连接酶催化亚基(GCLC)C-129T 多态性和修饰亚基(GCLM)G-23T 多态性与冠心病遗传易感性的关系。方法采用聚合酶链反应-限制性片段长度多态方法,检测212例冠心病与218例对照的 GCLC C-129T 和 GCLM G-23T 基因型分布及差异。结果冠心病组中 GCLC-129T 等位基因频率显著高于对照组(P<0.01),GCLC-129T 的冠心病发病风险是-129C 的2.38倍(95% CI:1.25～4.54)。与 GCLC-129CC 基因型相比,GCLC-129CT 基因型的冠心病发病风险显著增加至2.14倍(95% CI:1.08～4.24,P<0.05),GCLC-129T 等位基因携带者(CT、TT 基因型)患冠心病的风险显著增加至2.28倍(95% CI:1.16～4.49,P<0.05)。冠心病组中GCLM-23T 等位基因频率显著低于对照组(P<0.01),GCLM-23T 的冠心病发病风险是-23G 的0.59倍(95% CI:0.42～0.82)。与 GCLM-23GG 基因型相比,GT、TT 基因型和-23T 等位基因携带者(GT、TT 基因型)的冠心病发病风险分别为0.71倍(95% CI:0.47～1.08,P>0.05)、0.18倍(95%CI:0.06～0.55,P<0.01)和0.61倍(95% CI:0.42～0.92,P<0.05)。结论 GCLC C-129T 多态性可能是冠心病的一个遗传易感因素,而 GCLM G-23T 多态性可能是冠心病的一个遗传保护因素。
Objective To investigate the relationship between genetic polymorphisms of glutamylcysteine ligase catalytic subunit (GCLC) C-129T polymorphism and modified subunit (GCLM) G-23T polymorphism and genetic susceptibility to coronary heart disease. Methods The genotypes of GCLC C-129T and GCLM G-23T in 212 patients with coronary heart disease and 218 controls were detected by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). Results The frequency of GCLC-129T allele in coronary heart disease group was significantly higher than that in control group (P <0.01). The risk of coronary heart disease in GCLC-129T was 2.38 times (95% CI: 1.25-4.54) -129C. Compared with the GCLC-129CC genotype, the risk of coronary heart disease in GCLC-129CT genotype was significantly increased to 2.14 times (95% CI: 1.08-4.24, P <0.05). GCLC-129T allele carriers Genotype) significantly increased the risk of coronary heart disease to 2.28 times (95% CI: 1.16 to 4.49, P <0.05). The frequency of GCLM-23T allele in CHD group was significantly lower than that in control group (P <0.01). The risk of coronary heart disease in GCLM-23T was 0.59 times (95% CI: 0.42-0.82) -23G. Compared with the GCLM-23GG genotype, the risk of coronary heart disease was 0.71-fold (95% CI: 0.47-1.08, P> 0.05) for GT, TT genotype and -23T allele carriers (GT, TT genotype) ), 0.18-fold (95% CI: 0.06-0.55, P <0.01) and 0.61-fold (95% CI: 0.42-0.92, P <0.05). Conclusion GCLC C-129T polymorphism may be a genetic predisposition to coronary heart disease, while GCLM G-23T polymorphism may be a genetic protective factor in coronary heart disease.