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目的通过检测对比,检测DNA甲基转移酶DNMT 1及DNMT 3B在不同期别子宫内膜异位症(EMS)患者血液及腹腔液中的表达水平,探讨二者在EMS发病中的作用和影响。方法以84例行腹腔镜手术并证实为盆腔EMS的患者为研究对象。采用ELISA法检测84例EMS患者及38例对照者血清及腹腔液中DNMT 1、DNMT 3B的水平,分析二者的相关性。结果 EMS组血清中DNMT1、3B及腹腔液DNMT 3B水平显著高于非EMS组(P<0.05)。血清DNMT1、3B的表达水平在EMSⅢ~Ⅳ期高于Ⅰ~Ⅱ期与非EMS组,Ⅰ~Ⅱ期DNMT 3B亦较非EMS组高(P<0.05);腹腔液中DNMT 1、3B的表达水平在EMSⅠ~Ⅱ期高于Ⅲ~Ⅳ期与非EMS组,其中DNMT 3B在三组之间差异有统计学意义(P<0.05),而DNMT 1的组间差异无统计学意义(P>0.05)。增殖期腹腔液中DNMT 1水平高于分泌期(P<0.05)。血清中DNMT1与DNMT 3B表达呈现正相关关系,腹腔液中的DNMT 1与DNMT 3B呈正相关;血清与腹腔液中DNMT 3B呈正相关,DNMT 1在两样本中无统计学相关性(P>0.05)。结论甲基化改变是EMS发生发展的关键因素之一。DNMTs可以作为反映DNA甲基化状态的间接指标。
Objective To detect the expression levels of DNA methyltransferase DNMT 1 and DNMT 3B in the blood and peritoneal fluid of patients with different stages of endometriosis (EMS) through the detection and comparison, and to explore their roles and impact in the pathogenesis of EMS . Methods 84 patients undergoing laparoscopic surgery and confirmed as pelvic EMS patients for the study. The levels of DNMT 1 and DNMT 3B in serum and peritoneal fluid of 84 patients with EMS and 38 controls were detected by ELISA, and their correlations were analyzed. Results The serum levels of DNMT1 and 3B and DNMT 3B in peritoneal fluid in EMS group were significantly higher than those in non-EMS group (P <0.05). The expression of DNMT1,3B in serum was higher in EMS Ⅲ ~ Ⅳ than in Ⅰ ~ Ⅱ and non EMS groups, and DNMT 3B in Ⅰ ~ Ⅱ was also higher than that in non EMS group (P <0.05). DNMT 1,3 B expression in peritoneal fluid The levels in EMS Ⅰ ~ Ⅱ were higher than those in Ⅲ ~ Ⅳ and non-EMS groups, of which DNMT 3B had significant difference between the three groups (P <0.05), while there was no significant difference between DNMT 1 groups (P> 0.05). The level of DNMT 1 in proliferative phase was higher than that in secretory phase (P <0.05). There was a positive correlation between DNMT1 and DNMT3B expression in serum, DNMT1 and DNMT3B in peritoneal fluid were positively correlated, serum DNMT3B in peritoneal fluid was positively correlated, DNMT1 was not statistically significant between the two samples (P> 0.05) . Conclusion Methylation change is one of the key factors in the development of EMS. DNMTs can be used as an indirect indicator of DNA methylation status.