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目的:检测CD4+ CD25+ CD127low/-调节性T细胞(regulatory T cell,Treg)在甲状腺乳头状癌(papillary thyroid carcinoma,PTC)患者外周血的比例,探讨其临床意义。方法:选择2010年8月至2011年2月在上海交通大学医学院附属仁济医院进行手术治疗的PTC患者40例及甲状腺腺瘤患者30例,以CD4+ CD25+ CD127low/-为标志,采用流式细胞术检测PTC患者及甲状腺腺瘤患者外周血中CD4+ CD25+ CD127low/-Treg的比例,分析其与PTC临床病理特征的关系。结果:与甲状腺腺瘤患者比较,PTC患者外周血中CD4+ CD25+ CD127low/- Treg比例明显升高[(7.836±1.668)% vs(5.365±1.156)%,P<0.05];外周血CD4+ CD25+ CD127low/-Treg比例与PTC临床分期、颈部淋巴结转移相关(均P<0.05),与年龄、性别及肿瘤大小无关(P>0.05)。结论:PTC患者外周血CD4+ CD25+ CD127low/-Treg比例显著增加,且与PTC临床分期、颈部淋巴结转移相关。
Objective: To investigate the proportion of CD4 + CD25 + CD127low / - regulatory T cell (Treg) in peripheral blood of patients with papillary thyroid carcinoma (PTC) and to investigate its clinical significance. Methods: From August 2010 to February 2011, 40 patients with PTC and 30 patients with thyroid adenoma underwent surgery in Renji Hospital Affiliated to Shanghai Jiaotong University School of Medicine were enrolled in this study. The CD4 + CD25 + CD127low / The percentage of CD4 + CD25 + CD127low / -Treg in peripheral blood of patients with PTC and thyroid adenoma was detected by cytometry, and their relationship with the clinicopathological features of PTC was analyzed. Results: The proportion of CD4 + CD25 + CD127low / - Treg in peripheral blood of patients with PTC was significantly higher than that of patients with thyroid adenoma [(7.836 ± 1.668)% vs (5.365 ± 1.156)%, P <0.05] The proportion of Treg was correlated with clinical stage and lymph node metastasis (all P <0.05), but not with age, gender and tumor size (P> 0.05). Conclusion: The proportion of CD4 + CD25 + CD127low / -Treg in peripheral blood of patients with PTC is significantly increased, and it is correlated with clinical stage and lymph node metastasis of PTC.