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目的:对芫花中山柰酚-3-O-β-D-(6″-p-香豆酰)-吡喃葡萄糖苷(tiliroside)的含量进行测定。方法:采用反相高效液相色谱,ZORBAX XDB C18色谱柱(4.6 mm×250 mm,5μm);流动相为乙腈(A)-0.1%醋酸水溶液(B),梯度洗脱,0～18 min A:B 26∶74,18～33 mim A:B 80∶20;流速1.0 mL.min-1,检测波长310 nm,柱温25℃。结果:山柰酚-3-O-β-D-(6″-p-香豆酰)-吡喃葡萄糖苷在进样量0.1~5.0μg与峰面积呈良好的线性关系,r=0.999 9(n=6)。山柰酚-3-O-β-D-(6″-P-香豆酰)-吡喃葡萄糖苷的平均回收率101.50%,RSD 0.90%(n=6)。结论:该方法可准确地测定芫花中山柰酚-3-O-β-D-(6″-p-香豆酰)-吡喃葡萄糖苷,方法精密度高、简便、快速。
Objective: To determine the content of kaempferol-3-O-β-D- (6 “-p-coumaroyl) -glucoside in Daphne genkwa Flower.Methods: ZORBAX XDB C18 column (4.6 mm × 250 mm, 5 μm); mobile phase consisted of acetonitrile (A) -0.1% aqueous acetic acid (B), gradient elution 0-18 min A: B 26:74, 18-33 mim A: B 80:20, the flow rate was 1.0 mL.min-1, the detection wavelength was 310 nm and the column temperature was 25 ℃ .Results: The results showed that the concentration of kaempferol-3-O- Glucopyranoside in the injection volume of 0.1 ~ 5.0μg and the peak area showed a good linear relationship, r = 0.999 9 (n = 6). The average recovery of kaempferol-3-O-β-D- (6 ”-P-coumaroyl) -glucopyranoside was 101.50% with RSD 0.90% (n = 6) .Conclusion: The determination of kaempferol kaempferol -3-O-β-D- (6 "-p-coumaroyl) -glucopyranoside, the method of high precision, simple and rapid.