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目的建立莲蒿颗粒的薄层鉴别和含量测定方法。方法采用薄层色谱法(TLC)定性鉴别莲蒿颗粒中的一枝蒿、干姜及甘草;采用HPLC法测定制剂中荭草苷及一枝蒿酮酸的含量。结果莲蒿颗粒中一枝蒿、干姜及甘草的TLC图谱斑点清晰、重复性好、分离较好、专属性强、方法简便,阴性对照无干扰。荭草苷和一枝蒿酮酸的质量浓度分别在9.42~75.36(r=0.999 5)和4.83~77.28μg·mL-1(r=0.999 8)范围内呈良好的线性关系,平均加样回收率分别为100.47%和98.57%,RSD值分别为1.82%和1.38%;2个指标测定的精密度、重复性、稳定性和准确度实验RSD值均低于2.0%。结论该方法简便、快捷,结果准确、可靠,可作为该制剂的质量控制标准。
Objective To establish a thin layer identification and determination method of Artemisia selengensis. Methods TLC (TLC) was used to identify Artemisia annua, Rhizoma drynariae and Glycyrrhiza uralensis in Artemisia selengensis Turcz. Particles. HPLC was used to determine the content of the orientin and artesunate. Results TLC fingerprints of Artemisia annua, dried ginger and licorice in Artemisia selengensis Turcz. Particles were clear, reproducible, well-separated, strong specificity, simple and convenient method and non-interference in negative control. There was a good linear relationship between the concentrations of triterpenoid glycosides and artesunate in the range of 9.42-75.36 (r = 0.999 5) and 4.83-77.28 μg · mL-1 (r = 0.999 8), respectively. The average recovery rate RSD values of 1.82% and 1.38% were 100.47% and 98.57% respectively. The precision, repeatability, stability and accuracy of the two indexes were all lower than 2.0%. Conclusion The method is simple, rapid, accurate and reliable, and can be used as the quality control standard of the preparation.