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目的探讨环氧合酶-2(cyclooxygenase-2,COX-2)和表皮生长因子-2(v-erb-b2erythroblastic leukemia viral oncogene homolog2,HER-2/neu)蛋白在卵巢上皮性癌中的表达及其临床意义。方法利用免疫组化链酶素抗生物蛋白—过氧化物酶连接(streptavidin-perosidase,SP)法检测12例正常卵巢组织、18例卵巢良性上皮性肿瘤、10例卵巢交界性上皮性肿瘤和40例卵巢上皮性癌组织中COX-2和HER-2/neu蛋白的表达水平,并结合临床病理特征进行分析。结果①COX-2和HER-2/neu蛋白在卵巢正常组织、良性肿瘤、交界性肿瘤和上皮性癌中的阳性表达率分别为0%、5.6%、30.0%、85.O%和8.3%、11.1%、20.0%、52.5%,二者的表达率随肿瘤恶性程度增加而增加,差异有统计学意义(P<0.05)。②COX-2和HER-2/neu蛋白在卵巢上皮性癌中的表达均与临床分期、病理分级、组织学类型无关(P>0.05),与淋巴结转移有关(P<0.05),二者在卵巢上皮性癌组织中同时表达的阳性率为52.5%,呈正相关(r=0.44,P<0.05)。结论 COX-2和HER-2/neu蛋白与卵巢上皮性癌的发生发展密切相关,二者可能协同参与了卵巢癌的发生发展。
Objective To investigate the expression of cyclooxygenase-2 (COX-2) and epidermal growth factor-2 (HER-2 / neu) in ovarian epithelial carcinoma Its clinical significance. Methods Twelve cases of normal ovarian tissue, 18 cases of ovarian benign epithelial tumor, 10 cases of ovarian borderline epithelial tumor and 40 cases of ovarian borderline epithelial tumor were detected by immunohistochemical streptavidin-perosidase (SP) Cases of ovarian epithelial carcinoma COX-2 and HER-2 / neu protein expression levels, combined with clinical and pathological features were analyzed. Results ① The positive expression rates of COX-2 and HER-2 / neu protein in normal ovarian tissues, benign tumors, borderline tumors and epithelial carcinomas were 0%, 5.6%, 30.0%, 85.0% and 8.3% 11.1%, 20.0% and 52.5%, respectively. The expression rates of them both increased with the increase of the degree of malignancy (P <0.05). ② The expression of COX-2 and HER-2 / neu protein in epithelial ovarian cancer were not related to the clinical stage, pathological grade and histological type (P> 0.05), but also to the lymph node metastasis (P <0.05) The positive rate of concurrent expression in epithelial carcinoma was 52.5% (r = 0.44, P <0.05). Conclusion COX-2 and HER-2 / neu proteins are closely related to the occurrence and development of epithelial ovarian cancer, and both of them may participate in the development of ovarian cancer.