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目的研究中国南海侧扁软柳珊瑚Subergorgia suberosa的次级代谢产物。方法利用硅胶柱层析、Sephadex LH-20凝胶柱层析、HPLC等方法对化合物进行分离纯化;利用NMR,MS等波谱方法,并与文献对照,鉴定化合物的结构;利用抗菌活性模型和卤虫致死活性模型评价化合物的生物活性。结果从侧扁软柳珊瑚中分离鉴定了6个单体化合物,分别为:倍半萜化合物subergorgic acid(1),2-β-hydroxysubergorgic acid(2)和2-β-acetoxysubergorgic acid(3);甾体化合物3β-hydroxy-5α-pregnan-20-one(4),5α-pregnane-3,20-di-one(5)和胆甾醇(6)。结论化合物1～3对白色葡萄球菌Staphylococcus albus均显示出较强的抑制活性,化合物4对蜡状芽孢杆菌Bacillus cereus显示出较强的抑制活性,而化合物4和5显示出较强的卤虫致死活性。
Objective To study the secondary metabolites of Subergorgia suberosa in the South China Sea. Methods The compounds were isolated and purified by silica gel column chromatography, Sephadex LH-20 gel column chromatography and HPLC. The structures of the compounds were identified by NMR, MS and other spectroscopic methods. The antibacterial activity model and halogen The lethal activity model evaluates the biological activity of a compound. Results Six monomer compounds were isolated and identified from the coral of Phyllostachys praecox. They were subergorgic acid (1), 2-β-hydroxysubergorgic acid (2) and 2-β-acetoxysubergorgic acid The compounds 3β-hydroxy-5α-pregnan-20-one (4), 5α-pregnane-3,20-di-one (5) and cholesterol (6). Conclusion Compounds 1 to 3 showed strong inhibitory activity against Staphylococcus albus, while compound 4 showed stronger inhibitory activity against Bacillus cereus cereus, while compounds 4 and 5 showed stronger killing of Artemia active.