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芦荟(Aloe)是药用价值较大的多年生草本植物。为了提高它的繁殖速度,我们从1990年4月开始研究了芦荟腋芽的组织培养。芽的诱导是用不带叶片的0.5cm茎节为材料,用0.1％升汞消毒6分钟后接种在MS+ZEATIN0.1mg+TG0.05mg培养基中在25℃温度1500Lux光照条件下诱导生芽后转移到WHITE+吲哚丁酸0.2mg+NAA0.05mg+2.4-D0.05mg培养基中诱导生根,然后洗掉琼脂浸在KNOP10倍稀解液中浸24～28小时移栽到花盆培养士上驯化成苗。
Aloe is a medicinal herbaceous perennial herb. In order to improve its breeding speed, we started from April 1990 to study the axillary bud aloe tissue culture. The buds were induced from 0.5 cm stem segments without leaves and sterilized with 0.1% mercuric chloride for 6 minutes before being inoculated into MS + ZEATIN 0.1 mg + TG 0.05 mg medium for inducing shoots at 25 ° C and 1500 Lux irradiation After the transfer to WHITE + indole butyric acid 0.2mg + NAA0.05mg +2.4-D0.05mg medium induced rooting, and then washed off the agar immersed in KNOP10 times diluted solution for 24 to 28 hours immersed in pots cultivator Domestication into seedlings.