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目的对城市回用水的致突变性进行分析。方法于2005年9月(丰水期)和2006年4月(枯水期)采集处理前、后的城市回用水水样,利用固相萃取和气相色谱-质谱联用(GC-MS)技术测定城市回用水中16种多环芳烃(PAHs)的含量。采用Ames试验检测其致突变性,设2000、1000、500、250ml/皿(相当于原水的体积)4个剂量组,以及溶剂对照组和阳性对照组[敌克松(50μg/皿)]。结果丰水期和枯水期两次水样中均检测出PAHs,主要为芴和菲,其次为萘、苊、二氢苊、蒽、荧蒽、芘等。丰水期进水水样中16种PAHs的总浓度为1777.9ng/L,出水水样为1380.1ng/L;枯水期进水水样中16种PAHs的总浓度为2225.1ng/L,出水水样为1827.1ng/L。在无代谢活化系统条件下,丰水期进水水样对TA98菌株在2000ml/皿及TA100菌株在1000ml/皿时;出水水样对TA98在250ml/皿时,TA100菌株在500ml/皿时,回变菌落数均超过溶剂对照组1倍以上,且具有剂量-反应关系。枯水期进、出水样对TA98、TA100菌株在250ml/皿时,回变菌落数均超过溶剂对照组1倍以上,且具有剂量-反应关系。水样在2000ml/皿时,不同程度地出现抑菌现象。结论城市回用水含有一定量的PAHs,Ames试验表明城市回用水处理后并未完全去除有机污染物,仍具有致突变作用。
Objective To analyze the mutagenicity of urban reuse water. Methods Urban water samples were collected from urban areas before and after treatment in September 2005 (wet season) and April 2006 (dry season). The samples were determined by GC-MS and GC-MS 16 kinds of polycyclic aromatic hydrocarbons (PAHs) in reuse water. The Ames test was used to detect the mutagenicity. Four doses of 2000, 1000, 500, 250 ml / dish (equivalent to the volume of raw water), and the solvent control group and the positive control group [50 μg / dish] were tested. Results PAHs were detected in two water samples during the wet and dry periods, mainly fluorene and phenanthrene, followed by naphthalene, acenaphthene, dihydroanthracene, anthracene, fluoranthene and pyrene. The total concentration of 16 PAHs in influent samples was 1777.9 ng / L in wet season, and 1380.1 ng / L in effluent samples. The total concentration of 16 PAHs in influent samples during dry season was 2225.1 ng / L, 1827.1 ng / L. In the condition of no metabolic activation system, water samples of TA98 in 2000ml / dish and TA100 strains at 1000ml / dish when water samples were in wet season, TA98 at 250ml / dish in water sample, Replicate colonies were more than 1 times more than the solvent control group, and has a dose-response relationship. In the dry season, the effluent samples of TA98 and TA100 were more than 1 times of that of the solvent control group at the dose of 250ml / dish, and had a dose-response relationship. Water samples in 2000ml / dish, to varying degrees, antibacterial phenomenon. Conclusion The urban reuse water contains a certain amount of PAHs. The Ames test shows that municipal wastewater does not completely remove organic pollutants and still has mutagenic effects.