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目的 观察IL 6对前列腺癌细胞生长的影响 ,对雄激素依赖型和雄激素非依赖型前列腺癌细胞作用的差别。 方法 分别测定雄激素依赖的LNCaP细胞和雄激素非依赖的PC 3细胞产生IL 6的能力及外源性IL 6和抗IL 6抗体对此两种细胞生长的影响。 结果 LNCaP细胞几乎不产生IL 6 ,1ml(1 0 6 个 )PC 3细胞平均产生IL 6 1 90pg/ 4 8h ;外源性IL 6对LNCaP细胞生长有明显抑制作用 ,但对PC 3细胞的生长无明显作用 ;抗IL 6抗体能明显抑制PC 3细胞的生长 ,对LNCaP细胞生长无影响。 结论 PC 3细胞以自分泌的形式分泌大量IL 6 ,抗IL 6抗体的封闭作用可抑制PC 3细胞的生长。外源性IL 6能抑制LNCaP细胞的生长
Objective To observe the effect of IL 6 on the growth of prostate cancer cells and its effect on androgen-dependent and androgen-independent prostate cancer cells. Methods The ability of IL-6 production by androgen-independent LNCaP cells and androgen-independent PC 3 cells and the effects of exogenous IL 6 and anti-IL 6 antibodies on the growth of these two cells were determined. Results LNCaP cells almost did not produce IL-6. 1 ml (106) PC 3 cells produced an average of IL-6 1 90 pg / 48 h. Exogenous IL-6 significantly inhibited the growth of LNCaP cells, No significant effect; Anti-IL 6 antibody can significantly inhibit the growth of PC 3 cells, LNCaP cells had no effect on cell growth. Conclusions PC 3 cells secrete large amounts of IL 6 in an autocrine manner. The blocking effect of anti-IL 6 antibody can inhibit the growth of PC 3 cells. Exogenous IL 6 can inhibit the growth of LNCaP cells