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目的:观察补肾丹对衰老模型大鼠的学习记忆能力的影响。方法:将清洁级健康Wistar雄性大鼠,随机分为7组:正常对照组、金匮肾气丸组、补肾丹高、中、低剂量组、维生素E组、模型组。除正常对照组外各组腹腔注射D-gal(250mg·kg-1.d-1)60天,采用D-半乳糖致亚急性衰老法[1],造成大鼠拟衰老动物模型。同时,正常对照组和模型对照组给等体积的生理盐水,金匮肾气丸组同时给予金匮肾气丸(0.72g.kg-1.d-1)灌胃,补肾丹高、中、低剂量组同时分别给予相当于生药2.7、1.35、0.675g.kg-1.d-1灌胃。维生素E组每日灌服维生素E0.027g/kg,每日1次,共60天。各组大鼠分别进行水迷宫法、跳台法实验,比较各组动物潜伏期和正确反应次数。结果:补肾丹高、中、低剂量组动物行为学指标明显改善,与模型组比较差异有统计学意义(P<0.05)。结论:补肾丹能够显著改善大鼠的学习记忆能力。
Objective: To observe the effect of Bushen Dan on learning and memory abilities of aging rats. Methods: Wistar male Wistar rats were randomly divided into 7 groups: normal control group, Jinkui Shenqi Pill group, Bushen Dangao, middle and low dose group, vitamin E group and model group. In addition to the normal control group, D-gal (250 mg·kg-1.d-1) was intraperitoneally injected in each group for 60 days. Subacute aging was induced by D-galactose [1], resulting in a rat model of aging. At the same time, the normal control group and the model control group were given an equal volume of normal saline and the Jinkui Shenqi Pills group were given Jinkui Shenqi Pills (0.72g.kg-1.d-1) to gavage at the same time. The low-dose group was given intragastric administration of 2.7, 1.35, and 0.675 g.kg-1.d-1 equivalent to crude drugs. The vitamin E group was given daily dose of vitamin E 0.027 g/kg once a day for 60 days. Rats in each group were subjected to water maze and step-down experiments, respectively, and the incubation period and correct response times of each group of animals were compared. Results: The animal behavior indexes of the high-, middle-, and low-dose groups of Bushen Dan significantly improved, and there was a significant difference compared with the model group (P<0.05). Conclusion: Bushendan can significantly improve the learning and memory ability of rats.