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为了探讨苦参总碱、美洲大蠊提取物及两者联合用药对孕激素受体(PR)阴性的子宫内膜癌细胞株JEC的作用。笔者通过MTT法、流式细胞仪、Western blot检测苦参总碱、美洲大蠊提取物和两者联合用药后对JEC细胞的生长抑制、细胞周期及P53和c-erb B-2基因蛋白的表达影响。结果发现,1MTT法:苦参总碱和美洲大蠊提取物对JEC的生长均有抑制作用,且联合给药后抑制作用显著提高。2流式细胞仪分析:苦参总碱组细胞在G0/G1期的比例上升,美洲大蠊提取物组细胞在G2/M期比例上升,联合给药组的G0/G1期比例较其他2组显著上升,细胞生长受到抑制(P<0.05)。3Western blot法:苦参总碱组、美洲大蠊提取物组和联合给药组作用JEC细胞后,P53蛋白的表达水平上调,c-erb B-2蛋白表达水平下降(P<0.05)。以上结果说明苦参总碱、美洲大蠊提取物及联合给药对JEC细胞的生长均有抑制作用,且能通过上调P53和下调c-erb B-2蛋白的表达诱导细胞周期阻滞,抑制细胞增殖。
To investigate the effects of total alkaloids of Sophora flavescens, extracts of American cockroach, and their combination on the progesterone receptor (PR)-negative endometrial cancer cell line JEC. The authors used MTT assay, flow cytometry, and Western blot to detect the growth inhibition, cell cycle, and P53 and c-erb B-2 gene protein of JEC cells after combined treatment of total alkaloids of Sophora flavescens, American cockroach extract, and both. Expression of influence. As a result, it was found that 1MTT method: Sophora flavescens and Coix Americana extracts all inhibited the growth of JEC, and the inhibitory effect was significantly increased after combined administration. 2 Flow cytometry analysis: The proportion of cells in the total alkaloids of Sophora flavescens increased in the G0/G1 phase, the proportion of cells in the G. americana extract group increased in the G2/M phase, and the ratio of the G0/G1 phase in the combined administration group was higher than the other 2 The group significantly increased and cell growth was inhibited (P<0.05). 3Western blotting: After treated with JEC cells in the Sophora flavescens group, American cockroach extract group and co-administration group, the expression of P53 protein was up-regulated and the expression of c-erb B-2 protein was decreased (P<0.05). The above results showed that the total alkaloids of Sophora flavescens, extracts of Coix americana, and combined administration all had inhibitory effects on the growth of JEC cells, and could induce cell cycle arrest and inhibition by up-regulating P53 and down-regulating the expression of c-erb B-2 protein. Cell Proliferation.