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目的研究中缅边境微小按蚊分布和传疟作用。方法 2008年8月24日~9月12日选择中缅边境5个自然村,用诱蚊灯在人房和牛房捕蚊。以复合PCR鉴别微小按蚊A或C,以巢式PCR检测蚊体内的疟原虫感染率,以复合PCR鉴别微小按蚊腹血。结果复合PCR鉴定微小按蚊A型居多,占98.04%,疟原虫感染率为1.72%,人房人血指数为56.12%。结论此时间段缅甸克钦地区微小按蚊A型居多,且以吸人血为主,具有较高的疟疾传播潜势。
Objective To study the distribution and malaria transmission of Anopheles minimus in the border between China and Burma. Methods From August 24 to September 12, 2008, five natural villages along the border between China and Burma were selected, and mosquito lamps were used to catch mosquitoes in the human and cow houses. Anopheles minimus (A or C) was identified by multiplex PCR. The infection rate of malaria parasites in mosquitoes was detected by nested PCR and the differential blood samples were detected by multiplex PCR. Results The results of multiplex PCR showed that the majority of A type was Anopheles minimus, accounting for 98.04%. The infection rate of Plasmodium was 1.72% and the human blood index was 56.12%. Conclusions Most Anopheles minor mosquitoes type A in Kachin area of Burma during this time period are predominant, with predominantly suckable blood and high malaria transmission potential.