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目的：研究血管内皮生长因子（ＶＥＧＦ）、碱性成纤维细胞生长因子（ｂＦＧＦ）、转化生长因子－β１（ＴＧＦ－β１）及其受体和肿瘤源性粘附因子（ＴＡＦ）在骨肉瘤中的表达特点，以及它们与肿瘤内微血管密度和病人预后的关系。方法：应用免疫组化和形态计量检测８０例骨肉瘤微血管密度（ＭＶＤ），ＶＥＧＦ、ｂＦＧＦ、ＴＧＦ－β１及其受体和ＴＡＦ的表达，并应用ＣＯＸ比例风险模型检测上述因素和预后的关系。结果：８０例骨肉瘤中，ＶＥＧＦ／ＫＤＲ强阳性４２例（５２．５％），ｂＦＧＦ／ｂＦＧＦｒ阳性４６例（５７．５），ＴＧＦβ１／ＴＧＦβ（ＲＩ）阳性３１例（３８．８％），ＴＡＦ阳性３６例（４５％）。血管生成因子与其受体表达具有一致性，并与ＭＶＤ值显著相关。ＣＯＸ比例风险模型分析结果显示ＭＶＤ和ＶＥＧＦ是影响骨肉瘤的重要因素（Ｐ＜０．０５）。结论：ＶＥＧＦ、ｂＦＧＦ、ＦＧＦ－β１和ＴＡＦ是骨肉瘤血管生成的重要因子，并通过促进血管生成影响病人的预后。其中ＭＶＤ和ＶＥＧＦ是评估骨肉瘤病人预后的重要指标。
Objective: To study vascular endothelial growth factor (VEGF), basic fibroblast growth factor (bFGF), transforming growth factor-β1 (TGF-β1) and their receptors, and tumor-derived adhesion factor (TAF) in osteosarcoma Their expression characteristics, and their relationship with intratumoral microvessel density and patient prognosis. Methods: The expression of MVD, VEGF, bFGF, TGF-β1, their receptors and TAF in 80 patients with osteosarcoma were detected by immunohistochemistry and morphometry. The relationship between the above factors and prognosis was analyzed by COX proportional hazards model. RESULTS: Of the 80 osteosarcomas, 42 (52.5%) were strongly positive for VEGF/KDR, 46 (57.5) were positive for bFGF/bFGFr, and 31 (38.8%) were positive for TGFβ1/TGFβ (RI). TAF positive in 36 cases (45%). Angiogenic factors are consistent with their receptor expression and are significantly associated with MVD values. The results of the COX proportional hazards model showed that MVD and VEGF were important factors affecting osteosarcoma (P<0.05). CONCLUSIONS: VEGF, bFGF, FGF-β1 and TAF are important factors in the angiogenesis of osteosarcoma and affect the patient’s prognosis by promoting angiogenesis. MVD and VEGF are important indicators for evaluating the prognosis of patients with osteosarcoma.