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为了检测不同人群中CMV感染在流行病学上的差异,作者研究两组在CMV感染流行病学方式截然不同的人群中正常青年的LTT反应。一组为57名英国人,CMV感染多发于较大的儿童及青少年;另一组为33名伊拉克人,大部分CMV感染发生于婴儿期。取抗凝外周血10ml,用Ficoll-Triosil通过离心分出单个核细胞,用含15IU肝素和5%自体血浆的RPMI 1640培养液洗涤3次,调整细胞数至1×1×10~6/ml,取0.2ml,置于培养板中,同时加10μl CMV抗原AD169株(稀释度分别为0、1∶4、1∶16和
To examine the epidemiological differences of CMV infection in different populations, the authors studied LTT responses in normal youth in two groups of patients with distinct CMV epidemiological patterns. One group consisted of 57 Britons, with CMV infections occurring in larger children and adolescents; the other group consisted of 33 Iraqis, with the majority of CMV infections occurring in infancy. Take anticoagulant peripheral blood 10ml, mononuclear cells were separated by centrifugation with Ficoll-Triosil, washed three times with RPMI 1640 medium containing 15IU heparin and 5% autologous plasma to adjust the cell number to 1 × 1 × 10 ~ 6 / ml , Take 0.2ml, placed in the plate, while adding 10μl CMV antigen AD169 strains (dilution were 0,1: 4, 1:16 and