论文部分内容阅读
本研究采用胭脂红、柠檬黄混合食用色素诱发小鼠生殖细胞遗传性损伤，结果表明：不同剂量混合食用色素组诱发小鼠精子畸变率均高于蒸馏水组（ｐ＜０．０１），提示该混合食用色素可能引起生殖细胞遗传损伤。Ｐ＜０．０１从表１可看出，混合色素各剂量组及阳性对照组的精子畸形率与阴性对照组比较，均有高度显著意义（Ｐ＜０．０１），表明该混合物在不同剂量时，均对小鼠精子有一定的致畸作用，而各组与阳性对照比较可见，在低剂量时，与ＣＰ间有高度显著意义，高剂量（５００ｍｇ／ｋｇ）时已与ＣＰ无显著意义，表明该混合物达到一定剂量时对精子的毒性已接近阳性对照──环磷酰胺（ＣＰ）。另一方面，该混合色素也呈现出一定的剂量反应关系，结果见表２。表２胭脂红、柠檬黄混合染毒剂量与小鼠精子畸形率的关系＊统计处理采用２×Ｃ表线性回归分析，χ２＝１３０．７．Ｐ＜０．０１以上结果表明，该混合食用色素可能是一种诱变剂，促使精子生成过程中有关的基因发生突变而引起畸形，本研究中观察到的精子畸形主要是胖头、无钩、香蕉形等，而且还可观察到精子的不同发育阶段，赵氏等认为这是精原细胞后期或初级精原细胞早期对外来的化学诱变剂较为敏感［６］所致。同时，在研究中还可观察到不同剂量的?
In this study, the genetic damage of germ cells induced by mixed dietary pigment of carmine and lemon yellow was studied. The results showed that the mice sperm aberration induced by different doses of food dye group were higher than that of distilled water group (p <0.01) Mixed food coloring may cause germ cell genetic damage. P <0.01 As can be seen from Table 1, the mixed sperm of each dose group and the positive control group sperm deformity rate compared with the negative control group were highly significant (P <0.01), indicating that the mixture at different doses , All had certain teratogenic effects on mouse spermatozoa, while each group was compared with positive control. At low doses, there was a highly significant relationship with CP, and no significant difference with CP at high dose (500mg / kg) , Indicating that the sperm toxicity to the mixture at a given dose is approaching that of the positive control, cyclophosphamide (CP). On the other hand, the mixed pigment also showed a dose-response relationship, the results in Table 2. Table 2 Carmine, lemon yellow mixed dose dose and the relationship between sperm deformity * Statistical analysis using 2 × C table linear regression analysis, χ2 = 130.7. P <0.01 The above results show that the mixed food coloring may be a mutagen that causes mutations in the genes involved in spermatogenesis resulting in deformities. The sperm deformities observed in this study were mainly fat head, no hook , Banana-shaped, etc., but also observed at different stages of sperm development, Zhao et al think this is the early stage of spermatogonia or primary spermatogonia sensitive to exotic chemical mutagens [6] due. At the same time, different doses can be observed in the study?