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巨细胞病毒(CMV)长期被视为条件性致病病毒,受染者大多为亚临床型。对获得性感染,认为主要表现为肝病综合征。我们在对患病儿进行尿巨细胞包涵体细胞并临床研究的基础上,对少数患者作了 CMV—DNA 探针检测。现将结果报告如下。对象及方法对少数表现感染症状、尿内包涵体细胞阳性病例,用南通医学院研制的地高辛素巨细胞病毒 DNA 探针,以斑点杂交法对患儿尿样作核酸杂交检测。经点样、核酸变性、中和、预杂交、杂交等阶段,最后观察硝酸纤维素膜显
Cytomegalovirus (CMV) has long been regarded as a conditionally virulent virus, most of whom are subclinical. Acquired infection, said that the main manifestation of liver disease syndrome. We conducted a CMV-DNA probe assay on a small number of patients on the basis of a clinical study of urinary giant cell inclusion bodies in children. The results reported below. Subjects and Methods A small number of patients with symptoms of infection, urine inclusion body cells positive cases, with Nantong Medical College Digoxin cytomegalovirus DNA probe to spot hybridization of children with urine samples for nucleic acid hybridization detection. After spotting, nucleic acid denaturation, neutralization, pre-hybridization, hybridization and other stages, and finally observed nitrocellulose membrane was significantly